College of Environmental Science and Engineering, Ocean University of China, Qingdao 266100, China; Laboratory for Marine Ecology and Environmental Science, Pilot National Laboratory for Marine Science and Technology (Qingdao), Qingdao 266237, China; Key Laboratory of Marine Environment and Ecology, Ministry of Education, Ocean University of China, Qingdao 266100, China.
College of Marine Life Science, Ocean University of China, Qingdao 266003, China.
Sci Total Environ. 2021 Jan 1;750:142252. doi: 10.1016/j.scitotenv.2020.142252. Epub 2020 Sep 12.
The biogeography of active microbial communities and the underlying mechanisms in marine sediments are important in microbial ecology but remain unclear. Here, using qPCR and high-throughput sequencing, we investigated bacterial and archaeal community abundances and activities by quantifying the abundance and expression of the 16S rRNA gene respectively, RNA-derived bacterial and archaeal community biogeography, assembly mechanisms and co-occurrence relationships in surface sediment samples from the Bohai Sea (BS), South Yellow Sea (SYS) and the north East China Sea (NECS) of the eastern Chinese marginal seas. The results revealed a higher heterogeneity of bacterial and archaeal community activities than of abundances and heterogeneous ecological functions among areas reflected by community compositions. Furthermore, clear geographic groups (i.e., the BS, SYS and NECS groups) were observed for all, abundant and rare active bacterial and archaeal communities, accompanied by significant distance-decay patterns. However, the abundant and rare taxa showed inconsistent geographic patterns. More importantly, deterministic processes played a greater role than stochastic processes in active bacterial and archaeal community assembly. The rare taxa had weaker abilities to disperse and/or adapt and more complex ecological processes than the abundant taxa. In addition, this study also showed that intertaxa competition was the dominant interaction between active bacterial and archaeal members, which could greatly contribute to dispersal limitation. Moreover, active bacterial and archaeal co-occurrence patterns showed significant distance-decay patterns, which were consistent with the community compositions.
海洋沉积物中活性微生物群落的生物地理学及其潜在机制在微生物生态学中很重要，但仍不清楚。在这里，我们使用 qPCR 和高通量测序，通过分别定量 16S rRNA 基因的丰度和表达，分别量化了细菌和古菌群落的丰度和活性，研究了渤海（BS）、南黄海（SYS）和东海北部（NECS）表层沉积物样品中细菌和古菌群落的生物地理学、组装机制和共现关系。结果表明，与群落组成反映的区域相比，细菌和古菌群落的活性具有更高的异质性，生态功能也具有更高的异质性。此外，所有丰富和稀有活性细菌和古菌群落都观察到了明显的地理分组（即 BS、SYS 和 NECS 组），并伴随着显著的距离衰减模式。然而，丰富和稀有分类群显示出不一致的地理模式。更重要的是，确定性过程在活性细菌和古菌群落组装中比随机过程发挥更大的作用。稀有分类群的扩散和/或适应能力较弱，生态过程较复杂，而丰富分类群则相反。此外，本研究还表明，种间竞争是活性细菌和古菌成员之间的主要相互作用，这可以极大地促进扩散限制。此外，活性细菌和古菌的共现模式表现出显著的距离衰减模式，与群落组成一致。
