Liu Daoxin, Li Bin, Song Pengfei, Jiang Feng, Zhang Tongzuo
College of Agriculture and Animal Husbandry, Qinghai University, Xining 810016, China.
Key Laboratory of Adaptation and Evolution of Plateau Biota, Northwest Institute of Plateau Biology, Chinese Academy of Sciences, Xining 810001, China.
Microorganisms. 2024 Apr 12;12(4):789. doi: 10.3390/microorganisms12040789.
The gut microbiota in animals is a dynamic ecosystem influenced by both the host itself and the environment it inhabits. It is known that short-term captivity can significantly impact the gut microbiota of plateau zokors, leading to substantial inter-individual variation. However, the specific changes in the assembly process of the gut microbiota in plateau zokors during captivity remain unclear. In this study, we conducted a comparative analysis on the assembly process of the gut microbiota in 22 male plateau zokors from the same location in Qinglin Township, Datong County, Qinghai Province, before (W) and after (L) laboratory rearing. We performed a single-factor correlation network analysis on the top 50 genera with relative abundance in each group. The results revealed that captivity increased the complexity of the gut microbiota in plateau zokors, indicating a higher number of interactions between different microbial species. However, this increase in complexity was accompanied by a decrease in stability, suggesting a higher degree of variability and potential disruption in the microbial community. According to the results of the neutral community model, the gut microbiota of plateau zokors in the W had a higher Nm value (Nm = 48,135) compared to the L (Nm = 39,671), indicating that species dispersal of the gut microbiota was greater in the wild than in captivity. In the wild, the modified stochasticity ratio (MST) was less than 0.5, suggesting that deterministic processes dominated. However, after 15 days of laboratory rearing, the MST became greater than 0.5, indicating a shift toward stochastic processes, and this difference was highly significant ( < 0.001). This differs from research related to aboveground animals. This study provides theoretical support for the application of gut microbiota in subterranean endangered species.
动物的肠道微生物群是一个动态生态系统，受宿主自身及其栖息环境的影响。已知短期圈养会显著影响高原鼢鼠的肠道微生物群，导致个体间存在很大差异。然而，高原鼢鼠在圈养期间肠道微生物群组装过程的具体变化仍不清楚。在本研究中，我们对来自青海省大通县青林乡同一地点的22只雄性高原鼢鼠在实验室饲养前（W）和饲养后（L）的肠道微生物群组装过程进行了比较分析。我们对每组中相对丰度排名前50的属进行了单因素相关网络分析。结果表明，圈养增加了高原鼢鼠肠道微生物群的复杂性，这表明不同微生物物种之间的相互作用数量更多。然而，这种复杂性的增加伴随着稳定性的降低，这表明微生物群落的变异性和潜在破坏程度更高。根据中性群落模型的结果，W组高原鼢鼠的肠道微生物群Nm值（Nm = 48,135）高于L组（Nm = 39,671），这表明野生环境中肠道微生物群的物种扩散大于圈养环境。在野生环境中，修正随机比率（MST）小于0.5，这表明确定性过程占主导地位。然而，在实验室饲养15天后，MST大于0.5，这表明向随机过程转变，且这种差异非常显著（<0.001）。这与地上动物的相关研究不同。本研究为肠道微生物群在地下濒危物种中的应用提供了理论支持。
