School of Chemical Engineering, Northwest University, Xi'an 710069, China.
College of Food Science and Technology, Northwest University, Xi'an 710069, China.
Molecules. 2020 Nov 9;25(21):5211. doi: 10.3390/molecules25215211.
False indigo ( L., ) is the preferred tree indigenous for windbreak and sand control in Northwest China, while information on nutritional and bioactive characteristics of its honey is rare. Herein, 12 honey of L. (AFH) were sampled in Northwest China and the nutritional composition was determined. Sixteen mineral element and ten dominant polyphenols content were identified and quantified by ICP-MS (Inductively coupled plasma mass spectrometry) and HPLC-QTOF-MS (High performance liquid chromatography-Quadrupole time-of-flight mass spectrometry), respectively. Moreover, AFH demonstrated high levels of DPPH (1,1-Diphenyl-2-picrylhydrazyl) radical scavenging activity (IC50 100.41 ± 15.35 mg/mL), ferric reducing antioxidant power (2.04 ± 0.29 µmol FeSO·7HO/g), and ferrous ion-chelating activity (82.56 ± 16.01 mg NaEDTA/kg), which were significantly associated with total phenolic contents (270.07 ± 27.15 mg GA/kg) and ascorbic acid contents (213.69 ± 27.87 mg/kg). The cell model verified that AFH exhibited dose-dependent preventive effects on pBR322 plasmid DNA and mouse lymphocyte DNA damage in response to oxidative stress. Taken together, our findings provide evidence for the future application of AFH as a potential antioxidant dietary in food industry.
假木蓝(L.)是中国西北地区首选的防风固沙树种,但其蜂蜜的营养和生物活性特征信息却很少。本研究在中国西北地区采集了 12 种假木蓝蜂蜜(AFH),并测定了其营养成分。采用电感耦合等离子体质谱法(ICP-MS)和高效液相色谱-四极杆飞行时间质谱法(HPLC-QTOF-MS)分别鉴定和定量了 16 种矿物质元素和 10 种主要多酚类物质的含量。此外,AFH 表现出较高的 1,1-二苯基-2-苦基肼(DPPH)自由基清除活性(IC50 为 100.41 ± 15.35 mg/mL)、铁还原抗氧化能力(2.04 ± 0.29 μmol FeSO4·7H2O/g)和亚铁离子螯合能力(82.56 ± 16.01 mg EDTA-Na/kg),这与总酚含量(270.07 ± 27.15 mg GA/kg)和抗坏血酸含量(213.69 ± 27.87 mg/kg)显著相关。细胞模型验证了 AFH 对 pBR322 质粒 DNA 和小鼠淋巴细胞 DNA 氧化损伤具有剂量依赖性的预防作用。综上所述,本研究结果为 AFH 在食品工业中作为一种有潜力的抗氧化膳食补充剂的应用提供了依据。