Di Paola Monica, Seravalli Viola, Paccosi Sara, Linari Carlotta, Parenti Astrid, De Filippo Carlotta, Tanturli Michele, Vitali Francesco, Torcia Maria Gabriella, Di Tommaso Mariarosaria
Department of Biology, University of Florence, Sesto Fiorentino, 50019 Florence, Italy.
Department of Health Sciences, Division of Obstetrics & Gynecology, University of Florence, 50139 Florence, Italy.
J Clin Med. 2020 Nov 10;9(11):3621. doi: 10.3390/jcm9113621.
The vaginal microbiota plays a critical role in pregnancy. Bacteria from spp. are thought to maintain immune homeostasis and modulate the inflammatory responses against pathogens implicated in cervical shortening, one of the risk factors for spontaneous preterm birth. We studied vaginal microbiota in 46 pregnant women of predominantly Caucasian ethnicity diagnosed with short cervix (<25 mm), and identified microbial communities associated with extreme cervical shortening (≤10 mm). Vaginal microbiota was defined by 16S rRNA gene sequencing and clustered into community state types (CSTs), based on dominance or depletion of spp. No correlation between CSTs distribution and maternal age or gestational age was revealed. CST-IV, dominated by aerobic and anaerobic bacteria different than , was associated with extreme cervical shortening (odds ratio (OR) = 15.0, 95% confidence interval (CI) = 1.56-14.21; = 0.019). CST-III (-dominated) was also associated with extreme cervical shortening (OR = 6.4, 95% CI = 1.32-31.03; = 0.02). Gestational diabetes mellitus (GDM) was diagnosed in 10/46 women. Bacterial richness was significantly higher in women experiencing this metabolic disorder, but no association with cervical shortening was revealed by statistical analysis. Our study confirms that -depleted microbiota is significantly associated with an extremely short cervix in women of predominantly Caucasian ethnicity, and also suggests an association between -dominated microbiota (CST III) and cervical shortening.
阴道微生物群在妊娠中起着关键作用。来自某些菌属的细菌被认为可维持免疫稳态，并调节针对与宫颈缩短相关病原体的炎症反应，宫颈缩短是自发性早产的危险因素之一。我们研究了46名主要为白种人、被诊断为宫颈短（<25毫米）的孕妇的阴道微生物群，并确定了与极度宫颈缩短（≤10毫米）相关的微生物群落。通过16S rRNA基因测序定义阴道微生物群，并根据某些菌属的优势或缺失将其聚类为群落状态类型（CSTs）。未发现CSTs分布与产妇年龄或孕周之间存在相关性。以不同于某些菌属的需氧菌和厌氧菌为主的CST-IV与极度宫颈缩短相关（优势比（OR）=15.0，95%置信区间（CI）=1.56-14.21；P=0.019）。以某些菌属为主的CST-III也与极度宫颈缩短相关（OR = 6.4，95% CI = 1.32-31.03；P = 0.02）。46名女性中有10名被诊断为妊娠期糖尿病（GDM）。患有这种代谢紊乱的女性细菌丰富度显著更高，但统计分析未发现其与宫颈缩短有关联。我们的研究证实，在主要为白种人的女性中，某些菌属缺失的微生物群与极短的宫颈显著相关，并且还表明以某些菌属为主的微生物群（CST III）与宫颈缩短之间存在关联。
