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大豆木瓜蛋白酶样半胱氨酸蛋白酶的全基因组调查及其在根瘤共生中的表达分析。

Genome-wide survey of soybean papain-like cysteine proteases and their expression analysis in root nodule symbiosis.

机构信息

Key Laboratory of Biology and Genetic Improvement of Oil Crops, Ministry of Agriculture and Rural Affairs, Oil Crops Research Institute of Chinese Academy of Agricultural Sciences, Wuhan, China.

College of Resources and Environment, Fujian Agriculture and Forestry University, Fuzhou, 350002, Fujian, China.

出版信息

BMC Plant Biol. 2020 Nov 12;20(1):517. doi: 10.1186/s12870-020-02725-5.

DOI:10.1186/s12870-020-02725-5
PMID:33183238
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7659060/
Abstract

BACKGROUND

Plant papain-like cysteine proteases (PLCPs) are a large class of proteolytic enzymes and play important roles in root nodule symbiosis (RNS), while the whole-genome studies of PLCP family genes in legume are quite limited, and the roles of Glycine max PLCPs (GmPLCPs) in nodulation, nodule development and senescence are not fully understood.

RESULTS

In the present study, we identified 97 GmPLCPs and performed a genome-wide survey to explore the expansion of soybean PLCP family genes and their relationships to RNS. Nineteen paralogous pairs of genomic segments, consisting of 77 GmPLCPs, formed by whole-genome duplication (WGD) events were identified, showing a high degree of complexity in duplication. Phylogenetic analysis among different species showed that the lineage differentiation of GmPLCPs occurred after family expansion, and large tandem repeat segment were specifically in soybean. The expression patterns of GmPLCPs in symbiosis-related tissues and nodules identified RNS-related GmPLCPs and provided insights into their putative symbiotic functions in soybean. The symbiotic function analyses showed that a RNS-related GmPLCP gene (Glyma.04G190700) really participate in nodulation and nodule development.

CONCLUSIONS

Our findings improved our understanding of the functional diversity of legume PLCP family genes, and provided insights into the putative roles of the legume PLCPs in nodulation, nodule development and senescence.

摘要

背景

植物木瓜蛋白酶样半胱氨酸蛋白酶（PLCP）是一大类蛋白水解酶，在根瘤共生（RNS）中发挥重要作用，而豆类 PLCP 家族基因的全基因组研究相当有限，大豆 PLCPs（GmPLCPs）在结瘤、结节发育和衰老中的作用尚未完全了解。

结果

本研究鉴定了 97 个 GmPLCP，并进行了全基因组调查，以探索大豆 PLCP 家族基因的扩张及其与 RNS 的关系。通过全基因组复制（WGD）事件形成的 19 对基因组片段，包含 77 个 GmPLCP，鉴定为十九个基因复制对，显示出高度的复制复杂性。不同物种之间的系统发育分析表明，GmPLCP 的谱系分化发生在家族扩张之后，并且大豆中存在大量串联重复片段。共生相关组织和结节中 GmPLCP 的表达模式鉴定了与 RNS 相关的 GmPLCP，并为其在大豆中的共生功能提供了线索。共生功能分析表明，一个与 RNS 相关的 GmPLCP 基因（Glyma.04G190700）确实参与了结瘤和结节发育。

结论

我们的研究结果提高了我们对豆科 PLCP 家族基因功能多样性的认识，并为豆科植物 PLCP 在结瘤、结节发育和衰老中的潜在作用提供了线索。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/389e/7659060/eaf5bd365ec0/12870_2020_2725_Fig8_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/389e/7659060/a8e789018569/12870_2020_2725_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/389e/7659060/3f9e059187c2/12870_2020_2725_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/389e/7659060/da6dc3727d8d/12870_2020_2725_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/389e/7659060/82891ab6b5f3/12870_2020_2725_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/389e/7659060/8e590f00949d/12870_2020_2725_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/389e/7659060/252e250e5c95/12870_2020_2725_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/389e/7659060/5e98461fed65/12870_2020_2725_Fig7_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/389e/7659060/eaf5bd365ec0/12870_2020_2725_Fig8_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/389e/7659060/a8e789018569/12870_2020_2725_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/389e/7659060/3f9e059187c2/12870_2020_2725_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/389e/7659060/da6dc3727d8d/12870_2020_2725_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/389e/7659060/82891ab6b5f3/12870_2020_2725_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/389e/7659060/8e590f00949d/12870_2020_2725_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/389e/7659060/252e250e5c95/12870_2020_2725_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/389e/7659060/5e98461fed65/12870_2020_2725_Fig7_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/389e/7659060/eaf5bd365ec0/12870_2020_2725_Fig8_HTML.jpg

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