College of Food Science and Engineering, Ocean University of China, Qingdao, 266003, Shandong, PR China.
School of Medicine and Pharmacy, Ocean University of China, Qingdao, 266003, Shandong, PR China.
Carbohydr Polym. 2021 Jan 15;252:117139. doi: 10.1016/j.carbpol.2020.117139. Epub 2020 Sep 30.
Fucose-containing oligosaccharides (FCOs) have important applications in the food, medicine, and cosmetics industries owing to their unique biological activities. The degradation of microbial fucose-containing exopolysaccharide (FcEPS) is a promising strategy for obtaining FCOs, and bacteriophage-borne glycanase is a useful tool for degrading FcEPS. Here, we aimed to obtain FCOs using bacteriophage-borne glycanase to depolymerize FcEPS from Enterobacter sakazakii. The FcEPS was mainly composed of l-fucose (42.72 %), d-galactose (20.59 %), and d-glucose (21.81 %). Based on the results of nuclear magnetic resonance and mass spectrometry, the obtained FCOs were disaccharide fragments with backbones of β-d-Glcp-(1→4)-β-l-Fucp and α-d-Galp-(1→3)-β-l-Fucp, respectively. So far, few studies of disaccharides prepared from FcEPS have been reported. This study demonstrated that the FcEPS of E. sakazakii was a reliable fucose-containing disaccharide source and that bacteriophage-borne glycanase was an effective degradation tool for obtaining FCOs fragments from FcEPS.
岩藻糖基低聚糖（FCOs）具有独特的生物活性，在食品、医药和化妆品行业中有重要的应用。微生物岩藻糖基胞外多糖（FcEPS）的降解是获得 FCOs 的一种很有前途的策略，噬菌体携带的聚糖酶是降解 FcEPS 的有用工具。在这里，我们旨在使用噬菌体携带的聚糖酶从阪崎肠杆菌中获得 FCOs，以分解 FcEPS。FcEPS 主要由 l-岩藻糖（42.72%）、d-半乳糖（20.59%）和 d-葡萄糖（21.81%）组成。基于核磁共振和质谱的结果，得到的 FCOs 是具有β-d-Glcp-（1→4）-β-l-Fucp 和α-d-Galp-（1→3）-β-l-Fucp 骨架的二糖片段。到目前为止，很少有关于从 FcEPS 制备二糖的研究。本研究表明，阪崎肠杆菌的 FcEPS 是一种可靠的岩藻糖基二糖来源，噬菌体携带的聚糖酶是一种从 FcEPS 获得 FCOs 片段的有效降解工具。
