College of Food Science and Engineering, Ocean University of China, No. 5 Yushan Road, Qingdao, 266003, Shandong, People's Republic of China.
Arch Virol. 2021 Dec;166(12):3467-3472. doi: 10.1007/s00705-021-05255-z. Epub 2021 Oct 3.
The genome of a Cronobacter sakazakii M1 phage named PF-CE2 was characterized in this work, and a new species named "Cronobacter virus PF-CE2", in the genus Pseudotevenvirus of the subfamily Tevenvirinae of the family Myoviridae is proposed. The Gp190 gene of phage PF-CE2 is predicted to encode a bacteriophage-borne glycanase that is capable of degrading fucose-containing exopolysaccharides produced by C. sakazakii M1. Furthermore, we propose changing the taxonomic status of eight additional phages based on nucleotide sequence comparisons. This work provides a theoretical basis for subsequent heterologous expression of the phage PF-CE2 glycanase and provides an important reference for the preservation and sharing of these phages.
本工作对一株名为 PF-CE2 的 Cronobacter sakazakii M1 噬菌体基因组进行了研究，并提出了一个新的属“Cronobacter virus PF-CE2”，属于 Myoviridae 科 Tevenvirinae 亚科的 Pseudotevenvirus 属。噬菌体 PF-CE2 的 Gp190 基因预测编码一种噬菌体携带的糖苷酶，能够降解 Cronobacter sakazakii M1 产生的含有岩藻糖的胞外多糖。此外，我们还根据核苷酸序列比较，提出了另外 8 种噬菌体的分类地位变更建议。这项工作为后续噬菌体 PF-CE2 糖苷酶的异源表达提供了理论基础，为这些噬菌体的保存和共享提供了重要参考。
