Yin Zhen-Hua, Liu Xiao-Peng, Wang Jin-Mei, Xi Xue-Feng, Zhang Yan, Zhao Rui-Lin, Kang Wen-Yi
National R & D Center for Edible Fungus Processing Technology, Henan University, Kaifeng, China.
College of Physical Education, Henan University, Kaifeng, China.
Front Chem. 2022 Jan 27;10:825127. doi: 10.3389/fchem.2022.825127. eCollection 2022.
, a fungus, belongs to the Pleurotaceae family. The aim of the present study was to characterize the structure of a novel polysaccharide from fruiting bodies of (PPp-W) and evaluate its anticoagulant activity . The high-performance liquid chromatography and GC-MS analysis indicated that PPp-W with a molecular weight of 27.4 kDa was mainly composed of mannose (17.56%), glucose (6.37%), galactose (44.89%), and fucose (1.22%) with a certain amount of 3--methyled galactose. SEM, XRD, and AFM combined with Congo red test revealed that PPp-W was an irregular curly sheet with triple-helix conformation. The FT-IR, methylation, and nuclear magnetic resonance analysis indicated that PPp-W contained→6)-α-D-Gal-(1→, →6)-3--Me-α-D-Gal-(1→and →2, 6)-α-D-Gal-(1→ as main chain, partially substituted at -2 and -6 by non-reducing ends of βDMan-(1→ and βL-Fuc-(1→ with a small amount of α-1,3-linked-Glc in backbone. PPp-W could significantly prolong APTT (12.9 ± 0.42 s, < 0.001) and thrombin time (39.9 ± 0.28 s, < 0.01) compared with the control group (11.45 ± 0.071 s and 38.05 ± 0.21 s), which showed that PPp-W had anticoagulant activity. These studies suggested that PPp-W was a 3--methylated heteroglycan and might be suitable for functional foods and natural drugs as an anticoagulant ingredient, which provided a basis for the application of polysaccharides from .
[一种真菌]属于侧耳科。本研究的目的是表征从[该真菌]子实体中提取的一种新型多糖（PPp-W）的结构，并评估其抗凝血活性。高效液相色谱和气相色谱-质谱分析表明，分子量为27.4 kDa的PPp-W主要由甘露糖（17.56%）、葡萄糖（6.37%）、半乳糖（44.89%）和岩藻糖（1.22%）组成，并含有一定量的3-O-甲基化半乳糖。扫描电子显微镜、X射线衍射和原子力显微镜结合刚果红试验表明，PPp-W是具有三螺旋构象的不规则卷曲片层。傅里叶变换红外光谱、甲基化和核磁共振分析表明，PPp-W以→6)-α-D-半乳糖-(1→、→6)-3-O-甲基-α-D-半乳糖-(1→和→2,6)-α-D-半乳糖-(1→为主链，在-2和-6位部分被β-D-甘露糖-(1→和β-L-岩藻糖-(1→的非还原端取代，主链中含有少量α-1,3-连接的葡萄糖。与对照组（11.45±0.071 s和38.05±0.21 s）相比，PPp-W可显著延长活化部分凝血活酶时间（12.9±0.42 s，P<0.001）和凝血酶时间（39.9±0.28 s，P<0.01），表明PPp-W具有抗凝血活性。这些研究表明，PPp-W是一种3-O-甲基化杂聚糖，可能作为抗凝血成分适用于功能性食品和天然药物，为[该真菌]多糖的应用提供了依据。
