Department of Molecular Pharmacy, Faculty of Pharmacy, Masaryk University, Palackého tř. 1946/1, 612 42, Brno, Czech Republic; Department of Molecular Biology and Pharmaceutical Biotechnology, Faculty of Pharmacy, University of Veterinary and Pharmaceutical Sciences Brno, Palackého tř. 1946/1, 612 42, Brno, Czech Republic.
Department of Chemical Engineering, University of Chemistry and Technology, Prague, Technická 5, 166 28, Prague 6, Czech Republic.
Carbohydr Polym. 2021 Jan 15;252:117142. doi: 10.1016/j.carbpol.2020.117142. Epub 2020 Sep 28.
The goal of this work was to assess the usability of yeast glucan particles (GPs) as carriers for curcumin and determine the beneficial effect of a pharmacological composite of curcumin in GPs on dextran sulfate sodium induced colitis in rats. The assessment of the anti-inflammatory effect of particular substances was evaluated on the basis of the calculated disease activity index and by assessment of cytokines and enzymes from the gut tissue - tumor necrosis factor α (TNF-α), transforming growth factor β1, interleukin (IL)-1β, IL-6, IL-10, IL-17, catalase, superoxide dismutase 2, myeloperoxidase (MPO), and matrix metalloproteinase 9. Composites of GPs with incorporated curcumin showed promising results with the capability to lower symptoms of colitis and significantly decrease the production of pro-inflammatory cytokines TNF-α, IL-1β, IL-6, and the activity of MPO, as well. The anti-inflammatory effect of the composites was greater than those of pure GPs or curcumin.
本研究旨在评估酵母葡聚糖颗粒（GPs）作为姜黄素载体的可用性，并确定 GPs 中姜黄素药理复合物对葡聚糖硫酸钠诱导的大鼠结肠炎的有益作用。基于计算的疾病活动指数以及对肠道组织中细胞因子和酶的评估，评估了特定物质的抗炎作用 - 肿瘤坏死因子 α（TNF-α）、转化生长因子 β1、白细胞介素（IL）-1β、IL-6、IL-10、IL-17、过氧化氢酶、超氧化物歧化酶 2、髓过氧化物酶（MPO）和基质金属蛋白酶 9。掺入姜黄素的 GPs 复合材料表现出有希望的结果，能够降低结肠炎症状，并显著降低促炎细胞因子 TNF-α、IL-1β、IL-6 的产生以及 MPO 的活性。复合材料的抗炎作用大于纯 GPs 或姜黄素。
