Department of Molecular Biology and Pharmaceutical Biotechnology, Faculty of Pharmacy, University of Veterinary and Pharmaceutical Sciences Brno, Palackého tř. 1946/1, 612 42 Brno, Czech Republic.
Department of Chemical Engineering, University of Chemistry and Technology, Prague, Technická 5, 166 28 Prague, Czech Republic.
Int J Pharm. 2019 Sep 10;568:118532. doi: 10.1016/j.ijpharm.2019.118532. Epub 2019 Jul 16.
Glucan particles (GPs) from Saccharomyces cerevisiae are hollow shells that are composed mainly of β-1,3-d-glucan, which has demonstrated immunomodulatory and anti-inflammatory potential both in vitro and in vivo. Curcumin is a natural hydrophobic phenolic compound, which possesses a significant anti-inflammatory effect and is used as supportive therapy in the treatment of many inflammatory diseases. The aim of this study is to evaluate the possible synergic effect and other benefits of the co-application of GPs and curcumin in the form of pharmaceutical composites. GP/curcumin composites were prepared using controlled evaporation of the organic solvent and their anti-oxidative effect and anti-inflammatory potential were tested on THP1‑XBlue™‑MD2‑CD14 human monocytes cell line. The anti-oxidative effect was measured on pyocyanin-stimulated cells in vitro and the NF-κB/AP-1 signaling pathway on lipopolysaccharide pre-treated monocytes was chosen for anti-inflammatory assays. The secretion of pro-inflammatory cytokines TNF-α and IL-1β was evaluated as well. Results mostly showed a pro-oxidative activity of empty GPs, however, pharmaceutical composites demonstrated an anti-oxidative effect. The activity of NF-κB/AP-1 was substantially decreased by the tested GP/curcumin composites, which also caused the attenuation of cytokines secretion. The obtained results indicate a beneficial effect of the incorporation of curcumin into GPs.
来自酿酒酵母的葡聚糖颗粒 (GPs) 是由主要由β-1,3-d-葡聚糖组成的中空壳，已证明其具有体外和体内的免疫调节和抗炎潜力。姜黄素是一种天然疏水性酚类化合物，具有显著的抗炎作用，可作为许多炎症性疾病治疗的辅助疗法。本研究旨在评估 GPs 和姜黄素以药物复合材料形式共同应用的可能协同作用和其他益处。使用有机溶剂的控制蒸发制备了 GP/姜黄素复合材料，并在 THP1-XBlue™-MD2-CD14 人单核细胞系上测试了它们的抗氧化和抗炎潜力。在体外使用绿脓菌素刺激细胞测量抗氧化作用，并选择脂多糖预处理单核细胞的 NF-κB/AP-1 信号通路进行抗炎测定。还评估了促炎细胞因子 TNF-α和 IL-1β的分泌。结果主要显示空 GPs 的促氧化活性，然而，药物复合材料显示出抗氧化作用。测试的 GP/姜黄素复合材料显著降低了 NF-κB/AP-1 的活性，也导致细胞因子分泌减少。所得结果表明将姜黄素掺入 GPs 具有有益的效果。
