Biofilm Biology Laboratory, Centre for Research on Infectious Diseases (CRID), School of Chemical and Biotechnology, SASTRA Deemed University, Tirumalaisamudram, Thanjavur, 613 401 Tamil Nadu, India.
Pharmaceutical Technology Laboratory, School of Chemical and Biotechnology, SASTRA Deemed University, Thanjavur, 613 401 Tamil Nadu, India.
Carbohydr Polym. 2021 Jan 15;252:117192. doi: 10.1016/j.carbpol.2020.117192. Epub 2020 Oct 10.
Microorganisms which adhere to the surfaces of indwelling medical implants develop into a sessile microbial community to form monomicrobial or polymicrobial biofilms. Staphylococcus epidermidis and Candida albicans are the most common pathogens co-isolated from device mediated infections. Hence development of catheters coated with anti-fouling substances is of great interest. In this current study, chitosan, extracted from the shells of marine crab Portunus sanguinolentus was coated over the surface of the urinary catheters and checked for its efficacy to inhibit the adherence of both mono and mixed species biofilms. The Extracted Chitosan (EC) coated catheters showed profound activity in reducing the preformed biofilms and the other virulence factors of the pathogens like slime production in S. epidermidis and yeast to hyphal swtich in C. albicans. Furthermore, qPCR analysis showed that EC could downregulate the virulence genes in both the pathogens when grown as monospecies and mixed species biofilms.
定植于留置式医疗植入物表面的微生物会演变成定殖微生物群落，形成单种或多种微生物生物膜。表皮葡萄球菌和白色念珠菌是从器械介导感染中最常共同分离到的病原体。因此，开发具有抗污物质涂层的导管具有重要意义。在本研究中，从海洋蟹 Portunus sanguinolentus 的壳中提取的壳聚糖被涂覆在导尿管表面，以检查其抑制单种和混合物种生物膜附着的功效。提取的壳聚糖 (EC) 涂层导管在减少预形成的生物膜和病原体的其他毒力因子方面表现出显著的活性，如表皮葡萄球菌中的粘液产生和白色念珠菌中的酵母到菌丝的转变。此外，qPCR 分析表明，当 EC 作为单种和混合物种生物膜生长时，它可以下调两种病原体的毒力基因。
