Aghamolaei Somayeh, Behniafar Hamed, Behravan Mahmoodreza, Hajjaran Homa, Moin Vaziri Vahideh
Department of Parasitology and Mycology, School of Medicine, Student Research Committee, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Department of Basic Sciences, Medical Faculty of Sarab, Sarab, Iran.
J Parasit Dis. 2020 Dec;44(4):781-784. doi: 10.1007/s12639-020-01246-0. Epub 2020 Jul 23.
Leishmaniasis has an important impact on global public health, and the common form of the disease is cutaneous form as well in Iran. Different species of parasite make variable clinical manifestations, so prompt diagnosis and recognition at the species level are important due to their impact on the treatment and outcome of the disease. We aimed to examine the potential existence of the parasite genome in the exudate materials derived from lesions of the cutaneous leishmaniasis suspected patients referred to Varamin Health Center Laboratory, that were reported negative microscopically. Regarding the object of the study, kDNA-Nested-PCR was used. A 570 bp band equal to what expected for was amplified in 18 out of 29 tested samples (62%). Findings indicate the effectivness of kDNA as a high copy number gene to avoid false-negative results.
利什曼病对全球公共卫生有重要影响，在伊朗，该病的常见形式也是皮肤型。不同种类的寄生虫会导致不同的临床表现，因此由于其对疾病治疗和预后的影响，在物种水平上进行快速诊断和识别很重要。我们旨在检测转诊至瓦拉明健康中心实验室的疑似皮肤利什曼病患者病变渗出物中寄生虫基因组的潜在存在情况，这些患者显微镜检查报告为阴性。针对该研究目的，使用了kDNA巢式PCR。在29个检测样本中的18个（62%）中扩增出了一条570 bp的条带，与预期相符。研究结果表明kDNA作为高拷贝数基因可有效避免假阴性结果。
