Central Department of Biotechnology, Tribhuvan University, Kirtipur, Kathmandu, 44600, Nepal.
Sukraraj Tropical and Infectious Disease Hospital, Kathmandu, 44600, Nepal.
Sci Rep. 2023 Jul 29;13(1):12329. doi: 10.1038/s41598-023-37458-6.
Cutaneous leishmaniasis cases have increased dramatically in recent years in Nepal. The study offers molecular identification of the Leishmania species using 40 patient's aspiration biopsy samples, targeting markers kinetoplast minicircle DNA (kDNA) and internal transcribed spacer-1 (ITS1). Among molecularly diagnosed 22 cutaneous leishmaniasis cases, L. donovani complex was identified in 13 instances and L. major in 9 cases. The ITS1 PCR was positive in 12 of the positive nested- kDNA PCR cases (12/22), confirming L. donovani complex in seven of the cases and L. major in five of the cases. In addition, the study conclude that concurrent occurrence of atypical cutaneous infections caused by L. donovani parasite in 59.1% of cases and typical cutaneous infections caused by L. major parasite in 40.9% of cases. A Phylogentic analaysis showed that the detected L. donovani species present null genetic distances from seven references of L. donovani, but slight differences between ITS1 sequences and not grouped into a significant monophyletic cluster.
近年来，尼泊尔的皮肤利什曼病病例急剧增加。本研究使用 40 例患者的抽吸活检样本，针对动基体微环 DNA（kDNA）和内部转录间隔区-1（ITS1）标记物，对利什曼原虫种进行分子鉴定。在分子诊断的 22 例皮肤利什曼病病例中，13 例为杜氏利什曼原虫复合体，9 例为利什曼原虫。12 例阳性嵌套 kDNA PCR 病例中的 12 例（12/22）ITS1 PCR 阳性，证实 7 例为杜氏利什曼原虫复合体，5 例为利什曼原虫。此外，研究还得出结论，59.1%的病例同时存在由杜氏利什曼原虫寄生虫引起的非典型皮肤感染，40.9%的病例存在由利什曼原虫寄生虫引起的典型皮肤感染。系统发育分析显示，检测到的杜氏利什曼原虫种与 7 株杜氏利什曼原虫参考序列之间不存在遗传距离，但 ITS1 序列之间存在细微差异，未聚集成一个显著的单系群。
