Department of Periodontology, Faculty of Dentistry, Gazi University, Biskek caddesi 1.sokak 06500 Emek, Ankara, Turkey.
Department of Periodontology, Gulhane Faculty of Dental Medicine, Health Sciences University, Ankara, Turkey.
Clin Oral Investig. 2021 Jun;25(6):3555-3565. doi: 10.1007/s00784-020-03678-7. Epub 2020 Nov 12.
The aim of this study was to analyze the mRNA and protein expression of adiponectin, leptin, visfatin, tumor necrosis factor (TNF)-α, and interleukin (IL)-6 levels in periodontitis and peri-implantitis sites in systemically healthy individuals and to investigate the influence of the presence of current periodontitis on their expression levels in peri-implantitis sites.
Soft tissue biopsy samples were collected from 60 systemically healthy patients [15 periodontally healthy patients (group I), 16 patients with periodontitis (group II), 15 patients with peri-implantitis (group III), and 14 patients with peri-implantitis and periodontitis (group IV)]; mRNA expression levels of adiponectin, leptin, visfatin, TNF-α, and IL-6 were measured by quantitative real-time PCR; and their protein levels were assessed by immunohistochemistry.
The mRNA expression levels of all biomarkers were significantly higher for group II compared to group I, while significantly higher levels of leptin, TNF-α, and IL-6 were observed in group III in comparison with group I. Group II exhibited significantly higher mRNA expression of adiponectin and TNF-α than group III. Group IV showed significantly higher expression levels of adiponectin, leptin, TNF-α, and IL-6 compared to group III. Regarding the expression of protein levels, which was estimated through quantification of the histoscore, both groups II and III presented higher H-scores than group I for all biomarkers except leptin.
The presence of current periodontitis may enhance expression levels of adiponectin, leptin, TNF-α, and IL-6 in peri-implant soft tissue.
The presence of periodontitis is an important risk factor for the severity of peri-implant inflammation as well as the onset of peri-implantitis.
本研究旨在分析系统健康个体中牙周炎和种植体周围炎部位中脂联素、瘦素、内脂素、肿瘤坏死因子 (TNF)-α 和白细胞介素 (IL)-6 的 mRNA 和蛋白表达水平，并探讨现患牙周炎对种植体周围炎部位表达水平的影响。
从 60 名系统健康的患者中采集软组织活检样本[15 名牙周健康患者（I 组）、16 名牙周炎患者（II 组）、15 名种植体周围炎患者（III 组）和 14 名种植体周围炎和牙周炎患者（IV 组）]；通过实时定量 PCR 测量脂联素、瘦素、内脂素、TNF-α 和 IL-6 的 mRNA 表达水平；并通过免疫组织化学评估其蛋白水平。
与 I 组相比，所有生物标志物的 mRNA 表达水平在 II 组中均显著升高，而与 I 组相比，III 组中瘦素、TNF-α 和 IL-6 的水平显著升高。与 III 组相比，II 组的脂联素和 TNF-α mRNA 表达水平显著升高。与 III 组相比，IV 组的脂联素、瘦素、TNF-α 和 IL-6 表达水平显著升高。关于通过对组织评分进行量化来评估的蛋白水平，除了瘦素外，II 组和 III 组的所有生物标志物的 H 评分均明显高于 I 组。
现患牙周炎可能会增强种植体周围软组织中脂联素、瘦素、TNF-α 和 IL-6 的表达水平。
牙周炎的存在是种植体周围炎症严重程度以及种植体周围炎发生的重要危险因素。
