Department of Prosthodontics Faculty of Odontology, Rio de Janeiro State University, Rio de Janeiro, Brazil.
Department of Periodontology Faculty of Odontology, Rio de Janeiro State University, Rio de Janeiro, Brazil.
Clin Oral Investig. 2020 May;24(5):1837-1844. doi: 10.1007/s00784-019-03047-z. Epub 2019 Aug 23.
The aim of this study is to investigate the expression of sTREM-1 and its ligand PGLYRP1, as well as the expression of MMP-8 and its inhibitor TIMP-1, in peri-implant diseases. As a secondary aim, we analyzed the influence of the concomitant existence of periodontitis in the expression of these biomarkers.
This study included 77 patients (29 males and 48 females; mean age 55.0 ± 11.5), 18 having gingivitis, 16 having periodontitis, 20 having mucositis, and 23 having peri-implantitis. Patients were clinically examined, and unstimulated whole saliva was collected. sTREM-1, PGLYRP1, MMP-8, TIMP-1, and MMP-8/TIMP1 ratio were determined by ELISA.
The periodontitis group presented higher probing depth (PD) mean, and higher clinical attachment loss, compared with the other groups. The peri-implantitis group presented higher PD mean in implants compared to the mucositis group. Patients with PD ≥ 6 mm showed significantly higher levels of PGLYRP1, MMP-8, and MMP-8/TIMP-1 ratio than patients with PD < 6 mm. When all four markers were assessed, there were no significant differences between mucositis and peri-implantitis groups. Concomitant periodontitis resulted in higher significant levels of MMP-8 in patients with peri-implant disease.
We did not observe significant differences in the levels of the sTREM-1/PGLYRP1/MMP-8 axis between patients with periodontal and peri-implant diseases, suggesting that these markers are also involved in the inflammatory process around implants. Besides, the presence of periodontitis may affect the levels of MMP-8 in patients with peri-implant disease.
The sTREM-1/PGLYRP1/MMP-8 axis could be useful as potent markers in periodontal and peri-implant diseases.
本研究旨在探讨 sTREM-1 及其配体 PGLYRP1 以及 MMP-8 及其抑制剂 TIMP-1 在种植体周围病中的表达。作为次要目的，我们分析了同时存在牙周炎对这些生物标志物表达的影响。
本研究纳入了 77 名患者（29 名男性和 48 名女性；平均年龄 55.0±11.5 岁），其中 18 名患有牙龈炎，16 名患有牙周炎，20 名患有黏膜炎，23 名患有种植体周围炎。患者进行临床检查，并采集非刺激性全唾液。通过 ELISA 测定 sTREM-1、PGLYRP1、MMP-8、TIMP-1 和 MMP-8/TIMP1 比值。
与其他组相比，牙周炎组的探诊深度（PD）平均值和临床附着丧失更高。与黏膜炎组相比，种植体周围炎组的种植体 PD 平均值更高。PD≥6mm 的患者的 PGLYRP1、MMP-8 和 MMP-8/TIMP-1 比值明显高于 PD<6mm 的患者。当评估所有四个标志物时，黏膜炎和种植体周围炎组之间没有显著差异。同时患有牙周炎的患者，其 MMP-8 水平显著升高。
我们没有观察到牙周炎和种植体周围病患者之间 sTREM-1/PGLYRP1/MMP-8 轴水平有显著差异，这表明这些标志物也参与了种植体周围的炎症过程。此外，牙周炎的存在可能会影响种植体周围病患者 MMP-8 的水平。
sTREM-1/PGLYRP1/MMP-8 轴可能是牙周炎和种植体周围病的有用标志物。
