College of Light Industry and Food Engineering, Nanjing Forestry University, Nanjing, Jiangsu 210037, PR China.
School of Chemical Engineering and Technology, Tianjin University, Tianjin 300072, China.
J Agric Food Chem. 2020 Nov 25;68(47):13632-13640. doi: 10.1021/acs.jafc.0c04943. Epub 2020 Nov 13.
The involvement and the relationship between nitric oxide (NO) and hydrogen peroxide (HO) in methyl jasmonate (MeJA)-induced immune responses in blueberries against was explored using diphenylene iodonium (DPI, NADPH oxidase inhibitor) and 2-(4-carboxyphenyl)-4,4,5,5-tetramethylimidazoline-1-oxyl-3-oxide (cPTIO, NO scavenger). MeJA induced NO and HO burst and enhanced the resistance of blueberries by elevating defense-related enzymes and the phenylpropanoid pathway. However, the above impacts stimulated by MeJA were weakened by DPI and destroyed by cPTIO. Furthermore, cPTIO abolished the increment in HO by regulating the activities of NADPH oxidase, superoxide dismutase, catalase, and ascorbate peroxidase, whereas DPI weakened the increase in HO but barely affected the generation of NO and the activity of nitric oxide synthase elevated by MeJA. These results indicated that NO and HO were involved in the MeJA-induced resistance in blueberries, and NO worked upstream of HO in this process.
使用二苯基碘（DPI，NADPH 氧化酶抑制剂）和 2-（4-羧基苯基）-4,4,5,5-四甲基咪唑啉-1-氧-3-氧化物（cPTIO，NO 清除剂）探索了一氧化氮（NO）和过氧化氢（HO）在蓝莓对炭疽病的茉莉酸甲酯（MeJA）诱导免疫反应中的作用和关系。MeJA 诱导了 NO 和 HO 的爆发，并通过提高防御相关酶和苯丙烷途径增强了蓝莓的抗性。然而，DPI 和 cPTIO 削弱了 MeJA 刺激产生的上述影响。此外，cPTIO 通过调节 NADPH 氧化酶、超氧化物歧化酶、过氧化氢酶和抗坏血酸过氧化物酶的活性来消除 HO 的增加，而 DPI 减弱了 HO 的增加，但对 MeJA 升高的 NO 生成和一氧化氮合酶活性几乎没有影响。这些结果表明，NO 和 HO 参与了 MeJA 诱导的蓝莓抗性，并且在这个过程中，NO 在前 HO 起作用。
