College of Food Science and Nutritional Engineering , China Agricultural University , Beijing 100083 , China.
School of Agricultural Economics and Rural Development , Renmin University of China , Beijing 100872 , China.
J Agric Food Chem. 2018 Sep 26;66(38):9923-9932. doi: 10.1021/acs.jafc.8b03971. Epub 2018 Sep 18.
Methyl jasmonate (MeJA) and ethylene play important roles in mediating defense responses against Botrytis cinerea. Ethylene response factors (ERFs) are the final components of ethylene signal transduction; whether SlERF2 participates in disease resistance against Botrytis cinerea is unclear. The objective of this study was to investigate the role of SlERF2 in MeJA-mediated defense response by using both sense and antisense SlERF2 tomato fruit. Our results showed that both MeJA treatment and pathogen infection upregulated SlERF2 expression level. Overexpression of SlERF2 enhanced tomato fruit resistance against Botrytis cinerea. MeJA treatment increased ethylene production, promoted the activities of chitinase, β-1,3-glucanase, phenylalanine ammonia-lyase, and peroxidase, and elevated pathogenesis-related protein content and total phenolic content. Moreover, the effects of MeJA on disease response were reinforced in sense SlERF2 tomato fruit, while they were weakened in antisense SlERF2 tomato fruit. These results indicated that SlERF2 was involved in MeJA-mediated disease resistance against Botrytis cinerea in tomato fruit.
茉莉酸甲酯（MeJA）和乙烯在介导对灰葡萄孢的防御反应中起着重要作用。乙烯反应因子（ERFs）是乙烯信号转导的最终组成部分；SlERF2 是否参与对灰葡萄孢的抗病性尚不清楚。本研究旨在通过使用正反义 SlERF2 番茄果实来研究 SlERF2 在 MeJA 介导的防御反应中的作用。我们的结果表明，MeJA 处理和病原菌感染均上调 SlERF2 的表达水平。SlERF2 的过表达增强了番茄果实对灰葡萄孢的抗性。MeJA 处理增加了乙烯的产生，促进了几丁质酶、β-1,3-葡聚糖酶、苯丙氨酸解氨酶和过氧化物酶的活性，并提高了病程相关蛋白含量和总酚含量。此外，MeJA 对感病 SlERF2 番茄果实的抗病反应的影响增强，而对反义 SlERF2 番茄果实的影响减弱。这些结果表明，SlERF2 参与了番茄果实中 MeJA 介导的对灰葡萄孢的抗病性。
