Jarmusch Scott A, Lagos-Susaeta Diego, Diab Emtinan, Salazar Oriana, Asenjo Juan A, Ebel Rainer, Jaspars Marcel
Marine Biodiscovery Centre, Department of Chemistry, University of Aberdeen, Old Aberdeen AB24 3UE, Scotland, UK.
Mol Omics. 2021 Feb 1;17(1):95-107. doi: 10.1039/d0mo00084a. Epub 2020 Nov 13.
Siderophores are iron-chelating compounds that aid iron uptake, one of the key strategies for microorganisms to carve out ecological niches in microbially diverse environments. Desferrioxamines are the principal siderophores produced by Streptomyces spp. Their biosynthesis has been well studied and as a consequence, the chemical potential of the pathway continues to expand. With all of this in mind, our study aimed to explore extremotolerant and lupine rhizosphere-derived Streptomyces sp. S29 for its potential antifungal capabilities. Cocultivation of isolate S29 was carried out with Aspergillus niger and Botrytis cinerea, both costly fungal phytopathogens in the wine industry, to simulate their interaction within the rhizosphere. The results indicate that not only is Streptomyces sp. S29 extraordinary at producing hydroxamate siderophores but uses siderophore production as a means to 'starve' the fungi of iron. High resolution LC-MS/MS followed by GNPS molecular networking was used to observe the datasets for desferrioxamines and guided structure elucidation of new desferrioxamine analogues. Comparing the new chemistry, using tools like molecular networking and MS2LDA, with the known biosynthesis, we show that the chemical potential of the desferrioxamine pathway has further room for exploration.
铁载体是一类铁螯合化合物，有助于铁的摄取，这是微生物在微生物种类繁多的环境中开拓生态位的关键策略之一。去铁胺是链霉菌属产生的主要铁载体。其生物合成已得到充分研究，因此，该途径的化学潜力仍在不断扩展。考虑到所有这些因素，我们的研究旨在探索耐极端环境和羽扇豆根际来源的链霉菌S29的潜在抗真菌能力。将分离株S29与黑曲霉和灰葡萄孢进行共培养，这两种都是葡萄酒行业中造成巨大损失的真菌病原体，以模拟它们在根际中的相互作用。结果表明，链霉菌S29不仅在产生异羟肟酸铁载体方面表现出色，而且还利用铁载体的产生作为使真菌“缺铁”的一种手段。使用高分辨率液相色谱-串联质谱（LC-MS/MS）并结合全球天然产物社会分子网络（GNPS）来观察去铁胺的数据集，并指导新去铁胺类似物的结构解析。通过使用分子网络和MS2LDA等工具，将新化学物质与已知的生物合成进行比较，我们发现去铁胺途径的化学潜力还有进一步探索的空间。
