Aberer E, Stanek G
Department of Dermatology II, University of Vienna, Austria.
Am J Dermatopathol. 1987 Oct;9(5):374-9. doi: 10.1097/00000372-198710000-00002.
In order to elucidate the possible spirochetal origin of morphea and lichen sclerosus et atrophicans (LSA), we investigated biopsy specimens from 13 patients with morphea and 13 patients with LSA. Four patients with acrodermatitis chronica atrophicans (ACA), three with erythema chronicum migrans (ECM), and 11 patients with other inflammatory dermatoses served as controls. Formalin-fixed, paraffin-embedded sections were stained by an avidin-biotin-immunoperoxidase method, using serum of a patient with ACA that contained IgG antibodies to Borrelia burgdorferi. As positive control substrate, formalin-fixed culture suspensions of B. burgdorferi strain B31 were used. They presented either as thin, mostly linear, but partially dotted, stained spirochetes or, in contrast, as thick, "swollen," heavily stained organisms. Identical structures could also be detected on histological sections of one patient with ECM, four patients with morphea, and six patients with LSA. These findings provide evidence for the spirochetal origin of both morphea and LSA.
为了阐明硬斑病和硬化萎缩性苔藓（LSA）可能的螺旋体起源，我们研究了13例硬斑病患者和13例LSA患者的活检标本。4例进行性色素性紫癜性皮病（ACA）患者、3例慢性游走性红斑（ECM）患者以及11例其他炎症性皮肤病患者作为对照。用含有抗伯氏疏螺旋体IgG抗体的ACA患者血清，通过抗生物素蛋白-生物素免疫过氧化物酶方法对福尔马林固定、石蜡包埋的切片进行染色。作为阳性对照底物，使用伯氏疏螺旋体菌株B31的福尔马林固定培养悬液。它们呈现为纤细的、大多为线性但部分为点状的染色螺旋体，或者相反，呈现为粗大的、“肿胀的”、染色浓重的生物体。在1例ECM患者、4例硬斑病患者和6例LSA患者的组织学切片上也能检测到相同的结构。这些发现为硬斑病和LSA的螺旋体起源提供了证据。
