Department of Bacteriology, Mycology and Immunology, Faculty of Veterinary Medicine, Mansoura University, Mansoura, Egypt.
Educational Veterinary Hospital, Faculty of Veterinary Medicine, Mansoura University, Mansoura, Egypt.
J Appl Microbiol. 2021 Jul;131(1):485-498. doi: 10.1111/jam.14929. Epub 2020 Dec 5.
Infection of seafood with pathogenic species of the genus Vibrio causes human food-borne illnesses. This study was executed to examine the antimicrobial resistance phenotypes, biofilm-forming capability and virulence-associated genes of Vibrio from fish and shellfishes.
Three hundred fresh water and marine fish and shellfish samples were collected from wet markets and supermarkets in Mansoura, Egypt. Bacteriological examination and PCR amplification identified 92 Vibrio spp., including 42 Vibrio parahaemolyticus and 50 Vibrio alginolyticus isolates from the examined fish and shellfish (infection rate: 30·67%). However, V. vulnificus was not found in this study. Vibrio spp. exhibited variable frequencies of antimicrobial resistance with higher percentages to ampicillin and penicillin. Multidrug resistance (MDR) was detected in 69·04 and 38% of V. parahaemolyticus and V. alginolyticus respectively. PCR testing of virulence genes, tdh, trh and tlh revealed the presence of tlh and trh in 100 and 11·9% of V. parahaemolyticus isolates respectively and none of V. alginolyticus carried any of these genes. Biofilm-forming capability was displayed by 76% of V. parahaemolyticus and 73·8% of V. alginolyticus isolates. Both V. parahaemolyticus and V. alginolyticus showed nonsignificant weak positive correlations (r < 0·4) between antimicrobial pairs belonging to different classes; however, a significant positive correlation (P <0·05) between trh and resistance to erythromycin (r = 0·45) and imipenem (r = 0·38) was only identified in V. parahaemolyticus.
This study reports the existence of MDR strains of V. parahaemolyticus and V. alginolyticus from the common types of fishes and shellfishes in Egypt. Furthermore, the presence of virulence genes in these isolates and the ability to produce a biofilm in vitro pose potential health hazards to consumers.
Frequent monitoring of seafood for the presence of Vibrio spp. and their antimicrobial susceptibility, virulence determinants and biofilm-forming capability is important for assessing the risk posed by these organisms to the public and for improving food safety.
感染海产品的致病性弧菌属物种会导致人类食源性疾病。本研究旨在检测来自鱼类和贝类的弧菌的抗菌药物耐药表型、生物膜形成能力和毒力相关基因。
从埃及曼苏拉的湿市场和超市采集了 300 份淡水和海水鱼和贝类样本。细菌学检查和 PCR 扩增鉴定出 92 株弧菌属，包括从检查的鱼和贝类中分离出的 42 株副溶血性弧菌和 50 株 Algolytic 弧菌（感染率：30.67%）。然而，本研究中未发现创伤弧菌。弧菌属表现出不同频率的抗菌药物耐药性，对氨苄西林和青霉素的耐药率较高。69.04%和 38%的副溶血性弧菌和 Algolytic 弧菌分别表现出多药耐药（MDR）。PCR 检测毒力基因 tdh、trh 和 tlh 显示 100%的副溶血性弧菌携带 tlh 基因，11.9%的 Algolytic 弧菌携带 trh 基因，而 Algolytic 弧菌均不携带这些基因。76%的副溶血性弧菌和 73.8%的 Algolytic 弧菌能够形成生物膜。副溶血性弧菌和 Algolytic 弧菌之间的抗菌药物对之间仅存在非显著弱正相关（r<0.4），而 trh 与红霉素（r=0.45）和亚胺培南（r=0.38）的耐药性之间仅存在显著正相关（P<0.05）。
本研究报告了埃及常见鱼类和贝类中存在多药耐药副溶血性弧菌和 Algolytic 弧菌。此外，这些分离株存在毒力基因，并且能够在体外形成生物膜，这对消费者构成了潜在的健康危害。
定期监测海产品中弧菌属及其抗菌药物敏感性、毒力决定因素和生物膜形成能力的存在非常重要，这对于评估这些生物体对公众构成的风险和提高食品安全水平具有重要意义。
