State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei-MOST) & Key Laboratory of Oral Biomedicine Ministry of Education (KLOBM), School and Hospital of Stomatology, Wuhan University, Wuhan, China; Department of Oral and Maxillofacial Surgery, School and Hospital of Stomatology, Wuhan University, Wuhan, China.
State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei-MOST) & Key Laboratory of Oral Biomedicine Ministry of Education (KLOBM), School and Hospital of Stomatology, Wuhan University, Wuhan, China.
Oral Surg Oral Med Oral Pathol Oral Radiol. 2021 May;131(5):558-564. doi: 10.1016/j.oooo.2020.08.020. Epub 2020 Aug 26.
Increasing evidence indicates an interaction between the synovium and the cartilage in the temporomandibular joint (TMJ) and other joints. We recently demonstrated that the expression of proangiogenic factors was enhanced and that of factors promoting matrix degradation was decreased in synovial fibroblasts in condylar hyperplasia (CH). The aim of this study was to explore whether CH chondrocytes can affect the expression of these factors of synovial fibroblasts in a co-culture system.
The expressions of vascular endothelial growth factor (VEGF), cluster of differentiation 34 (CD34), fibroblast growth factor 2 (FGF-2), and tissue inhibitor of metalloproteinase 1 (TIMP1) from CH condylar tissues were observed by using immunohistochemical methods. Synovial fibroblasts of control tissues were co-cultured with the chondrocytes of CH, and protein expressions of VEGF, FGF-2, thrombospondin 1 (TSP1), matrix metalloproteinase 3 (MMP3), and TIMP1 were examined by using Western blotting.
Positive staining for VEGF, CD34, FGF-2, and TIMP1 was found in the hypertrophic cartilage layer of CH condylar tissues. Protein expressions of VEGF, FGF-2, and TIMP1 were significantly increased in co-cultured synovial fibroblasts, but TSP1 and MMP3 expressions were decreased.
The angiogenic factors and matrix degradation-related factors in synovial fibroblasts co-cultured with CH chondrocytes showed the same trends as those in synovial fibroblasts from CH tissue, suggesting potential cross-talk between synovial fibroblasts and chondrocytes during CH progression.
越来越多的证据表明，颞下颌关节（TMJ）和其他关节的滑膜与软骨之间存在相互作用。我们最近的研究表明，在髁突增生（CH）的滑膜成纤维细胞中，促血管生成因子的表达增强，而促进基质降解的因子的表达减少。本研究旨在探讨 CH 软骨细胞是否能在共培养系统中影响滑膜成纤维细胞这些因子的表达。
采用免疫组化方法观察 CH 髁状突组织中血管内皮生长因子（VEGF）、分化群 34（CD34）、成纤维细胞生长因子 2（FGF-2）和基质金属蛋白酶组织抑制剂 1（TIMP1）的表达。将对照组织的滑膜成纤维细胞与 CH 的软骨细胞共培养，用 Western blot 法检测 VEGF、FGF-2、血小板反应蛋白 1（TSP1）、基质金属蛋白酶 3（MMP3）和 TIMP1 的蛋白表达。
CH 髁状突组织肥大软骨层可见 VEGF、CD34、FGF-2 和 TIMP1 的阳性染色。共培养的滑膜成纤维细胞中 VEGF、FGF-2 和 TIMP1 的蛋白表达显著增加，而 TSP1 和 MMP3 的表达减少。
与 CH 软骨细胞共培养的滑膜成纤维细胞中的血管生成因子和基质降解相关因子的表达趋势与 CH 组织中的滑膜成纤维细胞相似，提示在 CH 进展过程中滑膜成纤维细胞和软骨细胞之间存在潜在的相互作用。
