Frederick National Laboratory for Cancer Research, Frederick, Maryland, USA.
Philadelphia College of Osteopathic Medicine-GA, Suwanee, Georgia, USA.
Cells Tissues Organs. 2024;213(3):245-254. doi: 10.1159/000532008. Epub 2023 Jul 31.
The impact of mild synovitis on the chondrogenic environment in the joint pertaining to cartilage repair is often neglected. In this study, 21 synovial samples were collected from foot surgeries for histology and isolation of fibroblast-like synoviocytes (FLSs). Of the 21 samples, 13 were normal and eight were mild synovitis, according to their synovitis scores. In mild synovitis, CD3+ lymphocytes were increased in the sublining layer. When chondrocytes were cultured and treated with the conditioned medium produced by FLSs, their glycosaminoglycan production was negatively correlated with the synovitis scores of the synovium, from which FLSs were isolated. In conclusion, mild synovitis in common joint conditions compromises the process of chondrogenesis, via inhibiting chondrocyte matrix production by FLSs. The results suggest that the concomitant synovitis, even being mild, could significantly alter the joint environment for chondrogenesis and impair the outcome of cartilage repair.
关节轻度滑膜炎对软骨修复中软骨内环境的影响往往被忽视。在这项研究中，我们从脚部手术中采集了 21 个滑膜样本，用于组织学和纤维母细胞样滑膜细胞（FLS）的分离。根据滑膜炎评分，21 个样本中 13 个为正常，8 个为轻度滑膜炎。在轻度滑膜炎中，滑膜下层的 CD3+淋巴细胞增加。当软骨细胞在 FLS 产生的条件培养基中培养并处理时，它们的糖胺聚糖产生与 FLS 分离的滑膜的滑膜炎评分呈负相关。总之，常见关节疾病中的轻度滑膜炎通过抑制 FLS 产生的软骨细胞基质来破坏软骨生成过程。这些结果表明，即使是轻度的伴随性滑膜炎也可能显著改变软骨生成的关节环境，并损害软骨修复的效果。
