Department of Bacteriology, Immunology, and Mycology, Faculty of Veterinary Medicine, Suez Canal University, Ismailia, 41522, Egypt.
Department of Medical Microbiology and Immunology, Faculty of Medicine, Assuit University, Assuit, 71515, Egypt.
Sci Rep. 2020 Nov 13;10(1):19779. doi: 10.1038/s41598-020-75914-9.
This study aimed to evaluate the prevalence, multidrug-resistance traits, PCR-detection of virulence, and antibiotic-resistance genes of E. coli isolated from secondary infections following FMD-outbreak in cattle. A total of 160 random samples were gathered from private dairy farms in Damietta Province, Egypt. The specimens were subjected to bacteriological examination, serotyping, congo-red binding assay, antibiogram-testing, and PCR-monitoring of virulence-determinant genes (tsh, phoA, hly, eaeA, sta, and lt) as well as the antibiotic-resistance genes (bla, bla, and bla). The prevalence of E. coli was 30% (n = 48) distributed in 8 serogroups (40/48, 83.3%), while 8 isolates (8/48, 16.6%) were untypable. Besides, 83.3% of the examined isolates were positive for CR-binding. The tested strains harbored the virulence genes phoA, hly, tsh, eaeA, sta, and lt with a prevalence of 100% and 50%, 45.8%, 25%, 8.4%, and 6.2%, respectively. Furthermore, 50% of the recovered strains were multidrug-resistant (MDR) to penicillins, cephalosporins, and carbapenems, and are harboring the bla, bla, and bla genes. Moreover, 25% of the examined strains are resistant to penicillins, and cephalosporins, and are harboring the bla and bla genes. To the best of our knowledge, this is the first report concerning the E. coli secondary bacterial infections following the FMD-outbreak. The emergence of MDR strains is considered a public health threat and indicates complicated treatment and bad prognosis of infections caused by such strains. Colistin sulfate and levofloxacin have a promising in vitro activity against MDR-E. coli.
本研究旨在评估口蹄疫疫情后牛继发性感染中分离的大肠杆菌的流行率、多药耐药特征、毒力 PCR 检测以及抗生素耐药基因。从埃及达米埃塔省的私人奶牛场随机采集了 160 份样本。对这些标本进行了细菌学检查、血清型鉴定、刚果红结合试验、药敏试验和毒力决定基因（tsh、phoA、hly、eaeA、sta 和 lt）以及抗生素耐药基因（bla、bla 和 bla）的 PCR 监测。大肠杆菌的流行率为 30%（n=48），分布在 8 个血清群中（40/48，83.3%），8 个分离株（8/48，16.6%）无法定型。此外，83.3%的受检分离株对 CR 结合呈阳性。测试菌株携带毒力基因 phoA、hly、tsh、eaeA、sta 和 lt，其流行率分别为 100%、50%、45.8%、25%、8.4%和 6.2%。此外，50%的回收菌株对青霉素、头孢菌素和碳青霉烯类药物具有多药耐药性（MDR），并携带 bla、bla 和 bla 基因。此外，25%的受检菌株对青霉素和头孢菌素耐药，并携带 bla 和 bla 基因。据我们所知，这是首例关于口蹄疫疫情后大肠杆菌继发性细菌感染的报告。MDR 菌株的出现被认为是对公共卫生的威胁，并表明此类菌株引起的感染治疗复杂且预后不佳。硫酸粘菌素和左氧氟沙星对 MDR-E. coli 具有良好的体外活性。
