Algammal Abdelazeem M, Enany Mohamed E, El-Tarabili Reham M, Ghobashy Madeha O I, Helmy Yosra A
Department of Bacteriology, Immunology and Mycology, Faculty of Veterinary Medicine, Suez Canal University, Ismailia 41522, Egypt.
Microbiology Department, Faculty of Science, Ain Shams University, Cairo 11556, Egypt.
Pathogens. 2020 May 9;9(5):362. doi: 10.3390/pathogens9050362.
Subclinical mastitis caused by has worldwide public health significance. Here, we aimed to determine the prevalence of , antimicrobial resistance profiles, and the virulence and enterotoxins determinant genes of MRSA strains that caused subclinical bovine mastitis. Milk samples were collected from 120 lactating animals (50 buffaloes and 70 dairy cattle) from different farms located in Ismailia Province (Egypt). The collected samples were investigated for subclinical mastitis using a California mastitis test. The total prevalence of was 35.9% (84/234) with 36.3% (53/146) in cattle and 31% (31/88) in buffaloes. Antimicrobial susceptibility testing showed that 35.7% (30/84) of the isolated strains were resistant to cefoxitin, defined as methicillin-resistant (MRSA), with 37.7% (20/53) in cattle and 32.2% (10/31) in buffaloes. Using PCR, 100% of the tested strains harbored and genes, while 86.6% were positive for gene, with remarkable gene size polymorphism. Additionally, 10% of the tested strains contained the gene. Further, using multiplex PCR, 26.6% of the tested samples had gene, two strains had gene and only one strain had and genes. The and genes were absent in the tested strains. In conclusion, and virulence genes were widely distributed in MRSA strains isolated from bovine milk, whereas the gene was the most predominant enterotoxin gene. Notably, this is the first report that emphasizes the prevalence of gene of MRSA isolated from bovine milk in Egypt.
由……引起的亚临床乳腺炎具有全球公共卫生意义。在此，我们旨在确定引起亚临床牛乳腺炎的耐甲氧西林金黄色葡萄球菌（MRSA）菌株的流行率、抗菌药物耐药谱以及毒力和肠毒素决定基因。从位于埃及伊斯梅利亚省不同农场的120头泌乳动物（50头水牛和70头奶牛）采集牛奶样本。使用加利福尼亚乳腺炎检测法对采集的样本进行亚临床乳腺炎调查。……的总流行率为35.9%（84/234），其中奶牛为36.3%（53/146），水牛为31%（31/88）。抗菌药物敏感性测试表明，35.7%（30/84）的分离菌株对头孢西丁耐药，被定义为耐甲氧西林金黄色葡萄球菌（MRSA），其中奶牛为37.7%（20/53），水牛为32.2%（10/31）。使用聚合酶链反应（PCR），100%的测试菌株携带……和……基因，而86.6%的菌株……基因呈阳性，基因大小存在显著多态性。此外，10%的测试菌株含有……基因。进一步使用多重PCR，26.6%的测试样本有……基因，两株有……基因，只有一株有……和……基因。测试菌株中不存在……和……基因。总之，……和……毒力基因在从牛乳中分离的MRSA菌株中广泛分布，而……基因是最主要的肠毒素基因。值得注意的是，这是第一份强调从埃及牛乳中分离的MRSA的……基因流行率的报告。
