Department of Entomology and Nematology, University of Florida, Gainesville, FL 32611, USA.
School of Biosciences, Cardiff University, Cardiff CF10 3AX, UK.
Database (Oxford). 2022 Apr 9;2022. doi: 10.1093/database/baac022.
Pesticidal proteins derived from the bacterium Bacillus thuringiensis, have provided the bases for a diverse array of pest management tools ranging from natural products used in organic agriculture, to modern biotechnological approaches. With advances in genome sequencing technologies and protein structure determination, an increasing number of pesticidal proteins from myriad bacterial species have been identified. The Bacterial Pesticidal Protein Resource Center (BPPRC) has been established to provide informational and analytical resources on the wide range of pesticidal proteins derived from bacteria that have potential utility for arthropod management. In association with a revised nomenclature for these proteins, BPPRC contains a database that allows users to browse and download sequences. Users can search the database for the best matches to sequences of interest and can incorporate their own sequences into basic informatic analyses. These analyses include the ability to draw and export guide trees from either whole protein sequences or, in the case of the three-domain Cry proteins, from individual domains. The associated website also provides a portal for users to submit protein sequences for naming. The BPPRC provides a single authoritative source of information to which all stakeholders can be referred including academics, government regulatory bodies and research and development personnel in the industrial sector. The database provides information on more than 1060 pesticidal proteins derived from 13 species of bacteria, including insecticidal activities for a subset of these proteins. Database URL: www.bpprc.org and www.bpprc-db.org/.
由细菌苏云金芽孢杆菌衍生的杀虫蛋白为多种多样的害虫管理工具提供了基础，这些工具包括有机农业中使用的天然产品，以及现代生物技术方法。随着基因组测序技术和蛋白质结构测定的进步，越来越多的来自各种细菌的杀虫蛋白已被鉴定。细菌杀虫蛋白资源中心（Bacterial Pesticidal Protein Resource Center，BPPRC）的建立旨在为源自具有节肢动物管理潜力的细菌的广泛杀虫蛋白提供信息和分析资源。与这些蛋白的修订命名法相关联，BPPRC 包含一个数据库，允许用户浏览和下载序列。用户可以在数据库中搜索与感兴趣序列最佳匹配的序列，并将自己的序列纳入基本的信息分析中。这些分析包括从全长蛋白序列或在三域 Cry 蛋白的情况下从单个结构域绘制和导出引导树的能力。相关网站还为用户提供了提交蛋白质序列进行命名的门户。BPPRC 为所有利益相关者提供了一个单一的权威信息来源，包括学术界、政府监管机构以及工业部门的研发人员。该数据库提供了来自 13 种细菌的 1060 多种杀虫蛋白的信息，其中包括这些蛋白中一部分的杀虫活性。数据库网址：www.bpprc.org 和 www.bpprc-db.org/。
