Caproni Leonardo, Raggi Lorenzo, Talsma Elise F, Wenzl Peter, Negri Valeria
Dipartimento di Scienze Agrarie, Alimentari e Ambientali (DSA3), Università Degli Studi Di Perugia, Borgo XX Giugno 74, 06126, Perugia, Italy.
Division of Human Nutrition and Health, Wageningen University and Research, PO Box 17, 6700 AA, Wageningen, The Netherlands.
Sci Rep. 2020 Nov 13;10(1):19775. doi: 10.1038/s41598-020-76417-3.
Mineral deficiencies represent a global challenge that needs to be urgently addressed. An adequate intake of iron and zinc results in a balanced diet that reduces chances of impairment of many metabolic processes that can lead to clinical consequences. In plants, bioavailability of such nutrients is reduced by presence of compounds such as phytic acid, that can chelate minerals and reduce their absorption. Biofortification of common bean (Phaseolus vulgaris L.) represents an important strategy to reduce mineral deficiencies, especially in areas of the world where this crop plays a key role in the diet. In this study, a panel of diversity encompassing 192 homozygous genotypes, was screened for iron, zinc and phytate seed content. Results indicate a broad variation of these traits and allowed the identification of accessions reasonably carrying favourable trait combinations. A significant association between zinc seed content and some molecular SNP markers co-located on the common bean Pv01 chromosome was detected by means of genome-wide association analysis. The gene Phvul001G233500, encoding for an E3 ubiquitin-protein ligase, is proposed to explain detected associations. This result represents a preliminary evidence that can foster future research aiming at understanding the genetic mechanisms behind zinc accumulation in beans.
矿物质缺乏是一个亟待解决的全球性挑战。摄入足够的铁和锌可使饮食均衡，减少许多可能导致临床后果的代谢过程受损的几率。在植物中，植酸等化合物的存在会降低此类营养素的生物有效性，这些化合物会螯合矿物质并减少其吸收。普通菜豆（Phaseolus vulgaris L.）的生物强化是减少矿物质缺乏的一项重要策略，特别是在世界上该作物在饮食中起关键作用的地区。在本研究中，对包含192个纯合基因型的多样性群体进行了铁、锌和植酸种子含量的筛选。结果表明这些性状存在广泛变异，并有助于鉴定合理携带有利性状组合的种质。通过全基因组关联分析，检测到锌种子含量与普通菜豆Pv01染色体上共定位的一些分子SNP标记之间存在显著关联。有人提出，编码E3泛素蛋白连接酶的基因Phvul001G233500可以解释所检测到的关联。这一结果是初步证据，有助于推动未来旨在了解菜豆锌积累背后遗传机制的研究。
