Burman S, Breslow E, Chait B T, Chaudhary T
Department of Biochemistry, Cornell University Medical College, New York.
Biochem Biophys Res Commun. 1987 Oct 29;148(2):827-33. doi: 10.1016/0006-291x(87)90950-8.
The original report assigning the pairing of neurophysin's 14 half-cystine residues (Schlesinger et al. (1972), Proc. Natl. Acad. Sci., U.S.A., 69,3350-3353) was based on an incorrect amino acid sequence. In the present study, re-investigation of the results of proteolytic fragmentation of bovine neurophysins indicates that the majority of the original assignments were incorrect. Three disulfide pairs are now assigned as Cys21-Cys44, Cys67-Cys85 and Cys74-Cys79. The pairing pattern indicates that neurophysin's variable carboxyl terminal region, separately encoded by the third gene exon, does not form a self-contained domain.
