克氏锥虫锥鞭毛体血流型被免疫 IgM 及其 Fab μ 片段转化为替代补体途径的激活剂。

Transformation of Trypanosoma cruzi trypomastigote bloodstream forms by immune IgM and its Fab mu fragment into activators of the alternative complement pathway.

Kipnis T L, Sucupira M, da Silva W D

Departamento de Imunologia, Universidade de São Paulo, Brasil.

Braz J Med Biol Res. 1987;20(1):105-14.

Abstract

We report that purified immune IgM obtained from Chagasic patients during the chronic stage of the disease and also immune IgM and its Fab mu fragments obtained from chronically T. cruzi-infected mice are capable of preparing trypomastigote forms of T. cruzi to be lysed by complement. 2. Mouse strains susceptible, moderately susceptible and resistant to T. cruzi infection are equally capable of producing antibodies with lytic activity as well as antibodies detectable by immunofluorescence and by immunoenzymatic assay. 3. The epitopes recognized by polyclonal lytic antibodies are present on the surface of trypomastigotes from many different strains and stocks of T. cruzi.

摘要

我们报告，从恰加斯病患者慢性期获得的纯化免疫IgM，以及从慢性感染克氏锥虫的小鼠获得的免疫IgM及其Fabμ片段，能够使克氏锥虫的无鞭毛体形式被补体裂解。2. 对克氏锥虫感染易感、中度易感和抗性的小鼠品系同样能够产生具有裂解活性的抗体，以及可通过免疫荧光和免疫酶测定法检测到的抗体。3. 多克隆裂解抗体识别的表位存在于来自许多不同克氏锥虫菌株和虫株的无鞭毛体表面。