比较针对克氏锥虫的鼠单克隆和多克隆抗体的 C 介导杀伤活性和 C 激活特性。

Comparison of the C-mediating killing activity and C-activating properties of mouse monoclonal and polyclonal antibodies against Trypanosoma cruzi.

机构信息

Departamento de Imunologia ICB Universidade de São Paulo Av. Prof. Lineu Prestes 2415 São Paulo CEP 05508-900 Brazil.

出版信息

Mediators Inflamm. 1992;1(5):309-12. doi: 10.1155/S0962935192000450.

DOI:10.1155/S0962935192000450

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC2365359/

Abstract

A Mouse polyclonal antiserum against Trypanosoma cruzi or its IgG and IgM fractions and five monoclonal antibodies (two IgM, two IgG(1) and one IgG(2a)) recognize and combine with membrane components of trypomastigote forms of the parasite as revealed by immunofluorescence. Although all these antibodies sensitize trypomastigotes and prepare them to activate the complement (C) system, as measured by consumption of total C, C4, B and C3, only the polyclonal antiserum or its IgG, IgM and Fabmu fragments were able to induce trypanosome lysis by the alternative C pathway.

摘要

一种针对克氏锥虫或其 IgG 和 IgM 部分的小鼠多克隆抗血清，以及五种单克隆抗体（两种 IgM、两种 IgG(1) 和一种 IgG(2a))，通过免疫荧光显示，可识别和结合寄生虫的锥虫体形式的膜成分。尽管所有这些抗体都能使锥虫体致敏并准备激活补体（C）系统，如通过总 C、C4、B 和 C3 的消耗来衡量，但只有多克隆抗血清或其 IgG、IgM 和 Fabmu 片段能够通过替代 C 途径诱导锥虫溶解。

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