Institute of Sustainable Processes, Dr. Mergelina s/n, 47011 Valladolid, Spain; Department of Chemical Engineering and Environmental Technology, School of Industrial Engineering, University of Valladolid, Dr. Mergelina, s/n, 47011 Valladolid, Spain.
Institute of Sustainable Processes, Dr. Mergelina s/n, 47011 Valladolid, Spain; Department of Chemical Engineering and Environmental Technology, School of Industrial Engineering, University of Valladolid, Dr. Mergelina, s/n, 47011 Valladolid, Spain.
Bioresour Technol. 2021 Jan;320(Pt B):124348. doi: 10.1016/j.biortech.2020.124348. Epub 2020 Nov 2.
The integral valorization of potential sugars (cellulosic and hemicellulosic) from spent coffee grounds (SCG), a lignocellulosic residue, is proposed in this work. With this aim, the microwave assisted dilute sulfuric acid pretreatment has been optimized, leading to a hemicellulosic sugar recovery in the pretreatment liquid (HSR) and an enzymatic hydrolysis yield of 79 and 98%, respectively, at 160.47 °C and 1.5% HSO. Moreover, the complete digestibility of cellulose (enzymatic hydrolysis yield = 100%) was also discovered for non-pretreated SCG, which is very interesting. Secondly, the production of biobutanol, an advanced biofuel, is also proposed from pretreated SCG enzymatic hydrolysate and pretreatment liquid achieved under optimal conditions. These were fermented by Clostridium beijerinckii, yielding 95 kg butanol/t SCG (dry matter) and 151 kg acetone-butanol-ethanol/t SCG (dry matter).
本工作提出了从咖啡渣（SCG）这一木质纤维素残渣中对潜在糖（纤维素和半纤维素）进行综合利用。为此，对微波辅助稀硫酸预处理进行了优化，在 160.47°C 和 1.5% HSO 下，分别得到预处理液中的半纤维素糖回收率（HSR）和 79%和 98%的酶水解产率。此外，还发现未经预处理的 SCG 的纤维素完全可消化（酶水解产率=100%），这非常有趣。其次，还提出了从最佳条件下获得的预处理 SCG 酶解液和预处理液中生产先进生物燃料丁醇。这些由拜氏梭菌发酵，每 SCG（干物质）生产 95kg 丁醇和 151kg 丙酮丁醇乙醇/t SCG（干物质）。
