Millar T J, Chubb I W
Department of Ophthalmology, Sydney Eye Hospital, University of Sydney, Woolloomooloo, N.S.W., Australia.
Brain Res. 1987 Sep 22;421(1-2):297-308. doi: 10.1016/0006-8993(87)91299-6.
The localization of acetylcholinesterase (AChE) in the chicken retina was studied using histochemical and immunohistochemical techniques. Using histochemistry, reaction end product was found in amacrine cells, ganglion cells, horizontal cells and in 4 bands in the inner plexiform layer. Ultrastructurally, the reaction end product was located between membranes of the endoplasmic reticulum, between the membranes of the nuclear envelope, surrounding neurites in the inner plexiform layer and filling synaptic clefts. Immunohistochemical techniques using a monoclonal antibody against AChE showed a similar staining pattern to that obtained with histochemistry. Ultrastructurally, AChE-like immunoreactivity was located on, not between, the membranes of the endoplasmic reticulum and nuclear envelope of amacrine cells, ganglion cells and horizontal cells. In the inner plexiform layer, immunoreactivity was on both pre- and postsynaptic membranes, and there was no immunoreactivity in non-terminal regions of the dendritic membranes and none within the synaptic clefts.
利用组织化学和免疫组织化学技术研究了鸡视网膜中乙酰胆碱酯酶（AChE）的定位。采用组织化学方法，在内侧无长突细胞、神经节细胞、水平细胞以及内侧丛状层的4条带中发现了反应终产物。在超微结构上，反应终产物位于内质网的膜之间、核膜之间、内侧丛状层的神经突周围以及突触间隙内。使用抗AChE单克隆抗体的免疫组织化学技术显示出与组织化学相似的染色模式。在超微结构上，AChE样免疫反应性位于无长突细胞、神经节细胞和水平细胞的内质网和核膜上，而非它们之间。在内侧丛状层，免疫反应性存在于突触前膜和突触后膜上，树突膜的非终末区域没有免疫反应性，突触间隙内也没有。
