Drujan B D, Díaz Borges J M, Brzin M
Can J Biochem. 1979 Jan;57(1):43-8. doi: 10.1139/o79-005.
The activity of cholinesterase and its cellular and subcellular localization were investigated in the retina and optic tectum of Eugerres plumieri and in the retina of Carassius carassius by means of radiometric, histochemical, and cytochemical procedures. In both fishes only the presence of acetylcholinesterase could be demonstrated. This study, besides confirming previous findings that acetylcholinesterase is located in the ganglion and amacrine cells of the retina as well as in the inner plexiform layer, in addition provides evidence that the enzyme is also present at the region of photoreceptor synapses between the cell bodies and apposing extensions of the horizontal cells of the same layer. The latter localization may indicate the involvement of a cholinergic mechanism at the functional contacts (transferapses) between the horizontal cells. In the optic tectum of Eugerres plumieri, histochemistry reveals fine distinguishable bands of acetylcholinesterase activity; two of the bands are quite sharply defined, whereas three others have rather a more diffuse appearance. The presence of these bands and their distribution may suggest a widespread distribution of cholinergic elements in the optic tectum.
采用放射测定法、组织化学法和细胞化学法，对银口钻光鱼视网膜和视顶盖以及鲫视网膜中胆碱酯酶的活性及其细胞和亚细胞定位进行了研究。在这两种鱼类中，仅证实存在乙酰胆碱酯酶。本研究除了证实先前的发现，即乙酰胆碱酯酶位于视网膜的神经节细胞和无长突细胞以及内网状层中之外，还提供了证据表明该酶也存在于同一层水平细胞的细胞体与相对延伸之间的光感受器突触区域。后一种定位可能表明胆碱能机制参与了水平细胞之间的功能性接触（传递突触）。在银口钻光鱼的视顶盖中，组织化学显示出乙酰胆碱酯酶活性的细微可区分条带；其中两条带相当清晰，而另外三条带的外观则较为弥散。这些条带的存在及其分布可能表明胆碱能元件在视顶盖中广泛分布。
