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源自C-KIT的环状RNA Hsa_circ_0069767上调对多发性骨髓瘤细胞生物学行为的影响

Effect of the Up-Regulation of Circular RNA Hsa_circ_0069767 Derived from C-KIT on the Biological Behavior of Multiple Myeloma Cells.

作者信息

Chen Fang, Wang Xiaohui, Fu Shuang, Wang Shaokun, Fu Yu, Liu Zhuogang, Zhang Jihong

机构信息

Hematology Laboratory, Shengjing Hospital of China Medical University, Shenyang 110022, People's Republic of China.

Department of Hematology, Shengjing Hospital of China Medical University, Shenyang 110022, People's Republic of China.

出版信息

Cancer Manag Res. 2020 Nov 6;12:11321-11331. doi: 10.2147/CMAR.S259393. eCollection 2020.

DOI:10.2147/CMAR.S259393
PMID:33192092
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7654535/
Abstract

PURPOSE

Multiple myeloma (MM) is an incurable disease. This study focused on the expression of circular RNA in MM and its influence on prognosis, in order to provide a potential target.

PATIENTS AND METHODS

Totally 66 MM patients participated in this research. Using RT-PCR method to determine the expression level of in 66 sorted samples from multiple myeloma patients and 21 normal control bone marrow samples, Kaplan-Meier was applied for survival analysis. We constructed stable over-expressing and silenced circ_0069767 cell lines and used MTS experiment to detect cell viability, transwell experiment to detect cell migration and invasion ability and flow cytometry to detect cell apoptosis. Dual luciferase experiment, qRT-PCR experiment and Western blot were used to explore miRNA and downstream genes.

RESULTS

The expression of in MM was significantly higher than that of the normal control group. Patients with high expression of had longer PFS and OS. Cell function experiments showed that overexpression of in MM cells led to decreased proliferation, migration and invasion, but increased apoptosis; meanwhile, knockdown of caused opposite biological behaviors. by sponging in MM cells regulates the expression of while the gene remained unmutated.

CONCLUSION

plays an antitumor role and its expression can be used as a reliable prognostic indicator for MM patients.

摘要

目的

多发性骨髓瘤（MM）是一种无法治愈的疾病。本研究聚焦于环状RNA在MM中的表达及其对预后的影响，以提供一个潜在靶点。

患者与方法

共有66例MM患者参与本研究。采用逆转录聚合酶链反应（RT-PCR）方法测定66例来自多发性骨髓瘤患者的分选样本及21例正常对照骨髓样本中[环状RNA名称未给出]的表达水平，应用Kaplan-Meier法进行生存分析。我们构建了稳定过表达和沉默circ_0069767的细胞系，并使用MTS实验检测细胞活力，transwell实验检测细胞迁移和侵袭能力，流式细胞术检测细胞凋亡。采用双荧光素酶实验、qRT-PCR实验和蛋白质免疫印迹法来探究微小RNA（miRNA）及其下游基因。

结果

MM中[环状RNA名称未给出]的表达显著高于正常对照组。[环状RNA名称未给出]高表达的患者无进展生存期（PFS）和总生存期（OS）更长。细胞功能实验表明，MM细胞中[环状RNA名称未给出]的过表达导致增殖、迁移和侵袭能力下降，但凋亡增加；同时，敲低[环状RNA名称未给出]则导致相反的生物学行为。在MM细胞中，[环状RNA名称未给出]通过海绵吸附[miRNA名称未给出]来调节[下游基因名称未给出]的表达，而[下游基因名称未给出]基因未发生突变。

结论

[环状RNA名称未给出]发挥抗肿瘤作用，其表达可作为MM患者可靠的预后指标。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/aed3/7654535/ac0d64f57c11/CMAR-12-11321-g0007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/aed3/7654535/4276a50f028c/CMAR-12-11321-g0001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/aed3/7654535/d66bc39636f3/CMAR-12-11321-g0002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/aed3/7654535/0bcff8905f9c/CMAR-12-11321-g0003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/aed3/7654535/ca495288063d/CMAR-12-11321-g0004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/aed3/7654535/b888cd3e877f/CMAR-12-11321-g0005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/aed3/7654535/8fd4fc8af430/CMAR-12-11321-g0006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/aed3/7654535/ac0d64f57c11/CMAR-12-11321-g0007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/aed3/7654535/4276a50f028c/CMAR-12-11321-g0001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/aed3/7654535/d66bc39636f3/CMAR-12-11321-g0002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/aed3/7654535/0bcff8905f9c/CMAR-12-11321-g0003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/aed3/7654535/ca495288063d/CMAR-12-11321-g0004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/aed3/7654535/b888cd3e877f/CMAR-12-11321-g0005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/aed3/7654535/8fd4fc8af430/CMAR-12-11321-g0006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/aed3/7654535/ac0d64f57c11/CMAR-12-11321-g0007.jpg

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2
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Aging (Albany NY). 2019 Dec 19;11(24):12043-12056. doi: 10.18632/aging.102539.
3
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Cancer Cell Int. 2023 Aug 26;23(1):178. doi: 10.1186/s12935-023-03028-z.
4
Clinical Significance of microRNAs in Hematologic Malignancies and Hematopoietic Stem Cell Transplantation.微小RNA在血液系统恶性肿瘤和造血干细胞移植中的临床意义
Cancers (Basel). 2023 May 8;15(9):2658. doi: 10.3390/cancers15092658.
5
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J Zhejiang Univ Sci B. 2022 Dec 15;23(12):975-988. doi: 10.1631/jzus.B2200211.
6
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7
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6
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7
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10
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