Mieszkowski Jan, Stankiewicz Błażej, Kochanowicz Andrzej, Niespodziński Bartłomiej, Borkowska Andżelika, Antosiewicz Jędrzej
Department of Gymnastics and Dance, Gdańsk, University of Physical Education and Sport, Gdańsk, Poland.
Department of Biomedical Basis of Physical Culture, Institute of Physical Education, Kazimierz Wielki University, Bydgoszcz, Poland.
Front Physiol. 2020 Oct 21;11:571220. doi: 10.3389/fphys.2020.571220. eCollection 2020.
Participation in a long-distance run, e.g., marathon or ultramarathon, continues to increase. One side effect of long-distance running is excessive inflammation manifested by the rise in inflammatory cytokine levels. We here aimed to elucidate the effects of 10-day ischemic preconditioning (IPC) training on marathon-induced inflammation and to evaluate the role of serum-stored iron in this process. The study involved 19 recreational runners taking part in a marathon. IPC training was performed in the course of four cycles, by inflating and deflating a blood pressure cuff at 5-min intervals (IPC group, = 10); the control group underwent sham training ( = 9). The levels of inflammatory and others markers (FSTL-1, IL-6, IL-15, leptin, resistin, TIMP-1, OSM, and LIF) were measured before and 24 h after training; and before, immediately after, and 24 h and 7 day after the marathon. The 10-day IPC training increased serum leptin levels. IL-6, IL-10, FLST-1, and resistin levels were increased, while TIMP-1 levels were decreased in all runners after the marathon. The changes were significantly blunted in runners from the IPC group compared with the control group. Baseline serum iron levels correlated with IL-6 and FSTL-1 levels; serum ferritin correlated with IL-6, FSTL-1, and resistin levels after the marathon. Conversely, serum TIMP-1 levels inversely correlated with serum iron levels. Although not evident at baseline, IPC training significantly reduced marathon-induced inflammation. In addition, the reduced responsiveness and attenuation of running-induced inflammation were inversely related to baseline serum iron and ferritin levels.
参加长跑（如马拉松或超级马拉松）的人数持续增加。长跑的一个副作用是炎症过度，表现为炎症细胞因子水平升高。我们旨在阐明10天缺血预处理（IPC）训练对马拉松运动引起的炎症的影响，并评估血清储存铁在这一过程中的作用。该研究纳入了19名参加马拉松的业余跑步者。IPC训练分四个周期进行，通过每隔5分钟给血压袖带充气和放气来完成（IPC组，n = 10）；对照组进行假训练（n = 9）。在训练前和训练后24小时；以及马拉松比赛前、比赛结束后即刻、比赛后24小时和7天，测量炎症及其他标志物（FSTL-1、IL-6、IL-15、瘦素、抵抗素、TIMP-1、OSM和LIF）的水平。为期10天的IPC训练使血清瘦素水平升高。马拉松赛后，所有跑步者的IL-6、IL-10、FLST-1和抵抗素水平均升高，而TIMP-1水平降低。与对照组相比，IPC组跑步者的这些变化明显减弱。基线血清铁水平与IL-6和FSTL-1水平相关；马拉松赛后血清铁蛋白与IL-6、FSTL-1和抵抗素水平相关。相反，血清TIMP-1水平与血清铁水平呈负相关。尽管在基线时不明显，但IPC训练显著减轻了马拉松运动引起的炎症。此外，跑步引起的炎症反应性降低和炎症减弱与基线血清铁和铁蛋白水平呈负相关。
