Wu Yinhua, Wang Zujin, Zhang Yu, Ruan Liming, Li Ang, Liu Xiaoyan
Department of Dermatology, The First Affiliated Hospital, School of Medicine of Zhejiang University, Hangzhou, China.
Department of the Second General Surgeon, The Yunhe People's Hospital, Yunhe, China.
Front Microbiol. 2020 Oct 19;11:548618. doi: 10.3389/fmicb.2020.548618. eCollection 2020.
Although the diversity and abundance of skin microbiome are mainly determined by intrinsic factors, including gender, age, anatomical site, and ethnicity, we question whether facial microbiome could be affected by long-term exposure to airborne pollution. Using 16S ribosomal RNA (rRNA) gene amplicon sequencing, we analyzed the facial bacterial microbiome of healthy and young Chinese women (25-35 years old) between two districts with different air quality indices (AQIs) in Zhejiang Province. The overall microbiome structure was obviously different between these two districts. It revealed an increase in both the abundance and diversity of facial bacterial microbiome in Hangzhou (HZ) with higher AQI compared with those in Yunhe (YH) with lower AQI. Linear discriminant analysis (LDA) and Lefse analysis identified a total of 45 genera showing significant overrepresentation in the HZ group. Furthermore, PICRUSt analysis showed that functional pathways associated with metabolism of saturated fatty acid were relatively more predominant in the HZ group, whereas those with DNA repair or mitochondrial DNA replication were more predominant in the YH group. Our present data can provide useful information for further researches on the composition and function of the skin microbiome related to air pollution factors as well as for the development of therapeutic agents targeting the microbes and their metabolites to resist damages of airborne pollutants.
虽然皮肤微生物群的多样性和丰度主要由内在因素决定，包括性别、年龄、解剖部位和种族，但我们质疑面部微生物群是否会受到长期暴露于空气污染物的影响。我们利用16S核糖体RNA（rRNA）基因扩增子测序，分析了浙江省两个空气质量指数（AQI）不同的地区中健康年轻中国女性（25 - 35岁）的面部细菌微生物群。这两个地区的整体微生物群结构明显不同。结果显示，与空气质量指数较低的云和（YH）地区相比，空气质量指数较高的杭州（HZ）地区面部细菌微生物群的丰度和多样性均有所增加。线性判别分析（LDA）和Lefse分析共鉴定出45个属在HZ组中显著富集。此外，PICRUSt分析表明，与饱和脂肪酸代谢相关的功能通路在HZ组中相对更为突出，而与DNA修复或线粒体DNA复制相关的功能通路在YH组中更为突出。我们目前的数据可为进一步研究与空气污染因素相关的皮肤微生物群的组成和功能提供有用信息，也可为开发针对微生物及其代谢产物的治疗药物以抵抗空气污染物的损害提供信息。
