Shao Li, Jiang Sujing, Li Yan, Shi Yanqin, Wang Man, Liu Ting, Yang Suzhen, Ma Laiji
School of Perfume and Aroma Technology, Shanghai Institute of Technology, Shanghai, People's Republic of China.
R&D Innovation Center, Shandong Freda Biotech Co., Ltd, Jinan, Shandong, People's Republic of China.
Clin Cosmet Investig Dermatol. 2022 Jun 7;15:1051-1063. doi: 10.2147/CCID.S364542. eCollection 2022.
Late bedtime is a common form of unhealthy sleep pattern in adulthood, which influences circadian rhythm, and negatively affects health. However, little is known about the effect of regular late bedtime on skin characteristics, particularly on skin microbiome.
To investigate the changes and effects of the regular late bedtime on skin physiological parameters and facial bacterial microbiome of 219 cases of Chinese women aged 18-38 years living in Shanghai.
Based on the Self-Evaluation Questionnaire, bedtime was categorized as 11:00 PM; thus, the volunteers were divided into early bedtime group (S0) and late bedtime group (S1). The physiological parameters of facial skin were measured by non-invasive instrumental methods, and the skin microbiome was analyzed by 16S rRNA high-throughput sequencing.
The skin physiological parameters of the late bedtime group exhibited significant decrease in skin hydration content, skin firmness (F4) and elasticity (R2), while TEWL, sebum and wrinkle significantly increased. The result indicated that late bedtime significantly impaired the integrity of skin barrier, damaged skin structure, and disrupted water-oil balance. Furthermore, the analysis of α-diversity, Sobs, Ace and Chao index were found to significantly decrease ( < 0.05) in the late bedtime group, suggesting that late bedtime reduced both the abundance and the diversity of facial bacterial microbiota. Moreover, the abundance of increased significantly, while , , and decreased significantly. In addition, Spearman correlation analysis revealed strong correlations between the microbiota and the physiological parameters. Notably, the abundance of significantly positively correlated with skin firmness and elasticity, but significantly negatively correlated with skin hemoglobin content, melanin content and skin hydration.
Bedtime is an important factor in maintaining skin health. Regular late bedtime not only damages the skin barrier and skin structure but also reduces the diversity and composition of facial bacterial microbiome.
晚睡是成年人不健康睡眠模式的常见形式，会影响昼夜节律，并对健康产生负面影响。然而，关于规律晚睡对皮肤特征，尤其是对皮肤微生物群的影响知之甚少。
探讨规律晚睡对219例居住在上海的18-38岁中国女性皮肤生理参数和面部细菌微生物群的变化及影响。
根据自我评估问卷，将就寝时间分为晚上11点；因此，志愿者被分为早睡组（S0）和晚睡组（S1）。采用非侵入性仪器方法测量面部皮肤的生理参数，并通过16S rRNA高通量测序分析皮肤微生物群。
晚睡组的皮肤生理参数显示皮肤水分含量、皮肤紧致度（F4）和弹性（R2）显著降低，而经皮水分流失、皮脂和皱纹显著增加。结果表明，晚睡显著损害了皮肤屏障的完整性，破坏了皮肤结构，并扰乱了水油平衡。此外，发现晚睡组的α多样性、Sobs、Ace和Chao指数显著降低（<0.05），表明晚睡降低了面部细菌微生物群的丰度和多样性。此外， 的丰度显著增加，而 、 、 和 显著降低。此外，Spearman相关性分析显示微生物群与生理参数之间存在强相关性。值得注意的是， 的丰度与皮肤紧致度和弹性显著正相关，但与皮肤血红蛋白含量、黑色素含量和皮肤水分显著负相关。
就寝时间是维持皮肤健康的重要因素。规律晚睡不仅会损害皮肤屏障和皮肤结构，还会降低面部细菌微生物群的多样性和组成。
