Animal Health Research Center (CISA-INIA), Madrid, Spain.
Biomedical Unit, School of Science, University of Waikato, Hamilton, New Zealand.
Front Immunol. 2020 Oct 30;11:596975. doi: 10.3389/fimmu.2020.596975. eCollection 2020.
In mammals, Blimp1 (B lymphocyte-induced maturation protein 1) encoded by the gene and its homolog Hobit (homolog of Blimp1 in T cells) encoded by , represent key transcriptional factors that control the development and differentiation of both B and T cells. Despite their essential role in the regulation of acquired immunity, this gene family has been largely unexplored in teleosts to date. Until now, one gene has been identified in most teleost species, whereas a homolog has not yet been reported in any of these species. Focusing our analysis on rainbow trout (), an identification and characterization of -like genes has been undertaken, confirming that and evolved from a common ancestor gene, acquiring three gene copies after the teleost-specific whole genome duplication event (WGD) and six genes after the salmonid-specific WGD. Additional transcriptional studies to study how each of these genes are regulated in homeostasis, in response to a viral infection or in B cells in different differentiation stages, provide novel insights as to how this gene family evolved and how their encoded products might be implicated in the lymphocyte differentiation process in teleosts.
在哺乳动物中，由 基因编码的 Blimp1（B 淋巴细胞诱导成熟蛋白 1）及其同源物 Hobit（T 细胞中的 Blimp1 同源物），代表了控制 B 和 T 细胞发育和分化的关键转录因子。尽管它们在获得性免疫的调节中具有重要作用，但迄今为止，该基因家族在硬骨鱼中基本上尚未得到探索。到目前为止，大多数硬骨鱼类中已经鉴定出一个 基因，而在这些鱼类中尚未报道任何 Hobit 同源物。我们将分析重点放在虹鳟鱼（）上，对 -样基因进行了鉴定和特征分析，证实 和 是从一个共同的祖先基因进化而来的，在硬骨鱼特异性全基因组复制事件（WGD）后获得了三个基因拷贝，在鲑鱼特异性 WGD 后获得了六个基因。进一步的转录研究旨在研究这些基因中的每一个在稳态、病毒感染或不同分化阶段的 B 细胞中是如何被调控的，这为该基因家族的进化以及它们编码的产物如何参与硬骨鱼淋巴细胞分化过程提供了新的见解。
