Morel Esther, Herranz-Jusdado J Germán, Simón Rocío, Abós Beatriz, Perdiguero Pedro, Martín-Martín Alba, Andrés Germán, Muñoz-Atienza Estefanía, Guerra Rodriguez Milagros, Díaz-Rosales Patricia, Tafalla Carolina
Animal Health Research Center (CISA-INIA), Valdeolmos, Madrid 28130, Spain.
Department of Genetics, Physiology and Microbiology, Faculty of Biological Sciences, Complutense University of Madrid (UCM), Madrid 28040, Spain.
iScience. 2022 Dec 21;26(1):105854. doi: 10.1016/j.isci.2022.105854. eCollection 2023 Jan 20.
The differentiation of B cells into antibody-secreting cells is fundamental for the generation of humoral immunity. In mammals, this process involves a series of metabolic and intracellular changes, not studied to date in teleost fish, where a clear distinction between naive B cells and plasmablasts/plasma cells (PCs) is still missing. Thus, in the current study, we have established that upon activation, teleost B cells undergo an expansion of the endoplasmic reticulum (ER) but experience no significant changes in mitochondria content. In parallel, the transcription of genes implicated in B cell differentiation increases, while that of mitochondrial genes decreases. In this context, ER monitoring has allowed us to distinguish between small cells with low amounts of ER (FSCER B cells), that correspond to undifferentiated cells, and large cells with expanded ER (FSCER B cells), characterized as plasmablasts. The results shed new light on the B cell differentiation process in teleosts and provide us with novel tools to study B cell function in these species.
B细胞分化为抗体分泌细胞是体液免疫产生的基础。在哺乳动物中，这一过程涉及一系列代谢和细胞内变化，而硬骨鱼迄今为止尚未对此进行研究，硬骨鱼中幼稚B细胞与成浆细胞/浆细胞（PC）之间仍缺乏明确区分。因此，在当前研究中，我们确定硬骨鱼B细胞激活后内质网（ER）会扩张，但线粒体含量无显著变化。同时，参与B细胞分化的基因转录增加，而线粒体基因转录减少。在此背景下，通过内质网监测，我们能够区分内质网含量低的小细胞（FSCER B细胞），其对应未分化细胞，以及内质网扩张的大细胞（FSCER B细胞），其特征为成浆细胞。这些结果为硬骨鱼的B细胞分化过程提供了新的见解，并为我们研究这些物种的B细胞功能提供了新工具。
