Kurisu K, Ohsaki Y, Nagata K, Kukita T, Yoshikawa H, Inai T
Second Department of Anatomy, Faculty of Dentistry, Kyushu University, Fukuoka, Japan.
Coll Relat Res. 1987 Oct;7(5):333-40. doi: 10.1016/s0174-173x(87)80026-2.
An immunocytochemical study was made on the palatal mesenchymal cells obtained from mouse embryos during palatal development with special reference to synthesis of types of collagen and fibronectin in vitro. Most cells showed positive staining with antibodies against the four proteins examined. The staining for type I collagen was most intense among the four proteins and was distributed perinuclearly. The staining for type III collagen was quite similar as that for type I collagen but less intense, whereas that for type V collagen was weak and its staining pattern was different from those for types I and III collagen in that the surface of the plasma membrane, in addition to the perinuclear cytoplasm, showed weak staining for type V collagen. Antibodies to fibronectin showed perinuclear and extracellular fibrous staining. These data suggest that palatal mesenchymal cells synthesize types I, III, and V collagen and fibronectin simultaneously.
对腭部发育期间从小鼠胚胎获取的腭间充质细胞进行了免疫细胞化学研究，特别关注体外胶原蛋白和纤连蛋白类型的合成。大多数细胞对所检测的四种蛋白质的抗体呈阳性染色。在这四种蛋白质中，I型胶原蛋白的染色最为强烈，且分布于细胞核周围。III型胶原蛋白的染色与I型胶原蛋白非常相似，但强度较弱，而V型胶原蛋白的染色较弱，其染色模式与I型和III型胶原蛋白不同，除了细胞核周围的细胞质外，质膜表面也显示出V型胶原蛋白的弱阳性染色。纤连蛋白抗体显示出细胞核周围和细胞外纤维状染色。这些数据表明腭间充质细胞同时合成I型、III型和V型胶原蛋白以及纤连蛋白。
