Ohsaki Y, Nagata K, Kurisu K
Second Department of Oral Anatomy, Kyushu University, Fukuoka, Japan.
Acta Anat (Basel). 1995;153(3):161-7. doi: 10.1159/000147696.
The detailed distribution of types I and III collagen and fibronectin was studied in the developing mouse palatal shelf using a double immunofluorescence technique. Just prior to palatal shelf elevation, type I collagen showed denser localization on the nasal side than on the oral side of the middle hard palate region. Such a distribution extended as far as the posterior hard palate region but not to the soft palate region. In type III collagen and fibronectin, this uneven distribution was not so distinct even in the middle hard palate region during the same period. The correspondence of the most conspicuous region of this distribution of extracellular matrix components with the region of first shelf elevation suggests that the extracellular matrix, especially type I collagen, participates in palate development.
利用双免疫荧光技术，研究了发育中小鼠腭突中I型和III型胶原蛋白及纤连蛋白的详细分布情况。就在腭突抬高之前，I型胶原蛋白在硬腭中部区域的鼻侧比口侧定位更密集。这种分布一直延伸到硬腭后部区域，但未延伸到软腭区域。在III型胶原蛋白和纤连蛋白中，即使在同一时期的硬腭中部区域，这种不均匀分布也不那么明显。细胞外基质成分这种分布最明显的区域与最初的腭突抬高区域相对应，这表明细胞外基质，尤其是I型胶原蛋白，参与了腭的发育。
