Silver M H, Foidart J M, Pratt R M
Differentiation. 1981;18(3):141-9. doi: 10.1111/j.1432-0436.1981.tb01115.x.
Indirect immunofluorescence has been used to study the distribution of fibronectin and collagen types I, II, and III in the developing primary and secondary palatal processes and forelimb buds of the Swiss Webster (NIH) mouse. In the palatal processes fibronectin and types I and III collagen are distributed throughout the mesenchyme. Fibronectin is present in the basement membrane, while types I and III collagen are localized in a linear, discontinuous fashion beneath the basement membrane. Fibronectin is not observed in the epithelium, including the presumptive fusion areas. In the forelimb bud these components show a similar distribution prior to chondrogenesis (early day 11). When chondrogenesis commences (late day 11 or early day 12) fibronectin and, to a lesser degree, types I and III collagen are apparently concentrated in the core mesenchyme, suggesting that fibronectin has a role in initiating chondrogenesis, perhaps by increasing cellular aggregation. Type II collagen is observed only in chondrogenic regions. The codistribution of fibronectin and types I and III collagen supports in vitro studies which indicate that cells use fibronectin to bind to collagen in the matrix. The developing chondrogenic regions appear to lose fibronectin gradually, concomitant with the appearance of type II collagen, suggesting that fibronectin is not involved in the maintenance of functional chondrocytes in their matrices.
间接免疫荧光法已被用于研究瑞士韦伯斯特（NIH）小鼠发育中的原发和继发腭突以及前肢芽中纤连蛋白和I、II、III型胶原蛋白的分布。在腭突中，纤连蛋白以及I型和III型胶原蛋白分布于整个间充质。纤连蛋白存在于基底膜中，而I型和III型胶原蛋白则以线性、不连续的方式定位于基底膜下方。在上皮中未观察到纤连蛋白，包括假定的融合区域。在前肢芽中，在软骨形成之前（第11天早期）这些成分显示出相似的分布。当软骨形成开始时（第11天晚期或第12天早期），纤连蛋白以及程度较轻的I型和III型胶原蛋白明显集中在核心间充质中，这表明纤连蛋白可能通过增加细胞聚集在启动软骨形成中发挥作用。仅在软骨形成区域观察到II型胶原蛋白。纤连蛋白与I型和III型胶原蛋白的共分布支持了体外研究，这些研究表明细胞利用纤连蛋白与基质中的胶原蛋白结合。随着II型胶原蛋白的出现，发育中的软骨形成区域似乎逐渐失去纤连蛋白，这表明纤连蛋白不参与功能性软骨细胞在其基质中的维持。
