Magno Luis M, Hinds David T, Duffy Paul, Yadav Rahul B, Ward Andrew D, Botchway Stan W, Colavita Paula E, Quinn Susan J
School of Chemistry, University College Dublin, Dublin, Ireland.
School of Chemistry, Trinity College Dublin, Dublin, Ireland.
Front Chem. 2020 Oct 14;8:576175. doi: 10.3389/fchem.2020.576175. eCollection 2020.
In this study the application of porous carbon microparticles for the transport of a sparingly soluble material into cells is demonstrated. Carbon offers an intrinsically sustainable platform material that can meet the multiple and complex requirements imposed by applications in biology and medicine. Porous carbon microparticles are attractive as they are easy to handle and manipulate and combine the chemical versatility and biocompatibility of carbon with a high surface area due to their highly porous structure. The uptake of fluorescently labeled microparticles by cancer (HeLa) and normal human embryonic Kidney (HEK 293) cells was monitored by confocal fluorescence microscopy. In this way the influence of particle size, surface functionalization and the presence of transfection agent on cellular uptake were studied. In the presence of transfection agent both large (690 nm) and small microparticles (250 nm) were readily internalized by both cell lines. However, in absence of the transfection agent the uptake was influenced by particle size and surface PEGylation with the smaller nanoparticle size being delivered. The ability of microparticles to deliver a fluorescein dye model cargo was also demonstrated in normal (HEK 293) cell line. Taken together, these results indicate the potential use of these materials as candidates for biological applications.
在本研究中，展示了多孔碳微粒在将难溶性物质转运到细胞中的应用。碳提供了一种本质上可持续的平台材料，能够满足生物学和医学应用所带来的多重复杂要求。多孔碳微粒具有吸引力，因为它们易于处理和操控，并且由于其高度多孔的结构，将碳的化学多功能性和生物相容性与高表面积结合在一起。通过共聚焦荧光显微镜监测癌细胞（HeLa）和正常人类胚胎肾细胞（HEK 293）对荧光标记微粒的摄取。通过这种方式，研究了粒径、表面功能化和转染剂的存在对细胞摄取的影响。在转染剂存在的情况下，两种细胞系都能轻易内化大微粒（690 nm）和小微粒（250 nm）。然而，在没有转染剂的情况下，摄取受到粒径和表面聚乙二醇化的影响，较小的纳米颗粒尺寸更易被摄取。在正常（HEK 293）细胞系中也证明了微粒递送荧光素染料模型货物的能力。综上所述，这些结果表明这些材料作为生物应用候选物的潜在用途。
