Schmidt Cristine, Bovolini José A, Gonçalves Nádia, Vasques-Nóvoa Francisco, Andrade Maria do Amparo, Santos Mário, Leite-Moreira Adelino, Henriques-Coelho Tiago, Duarte José Alberto, Moreira-Gonçalves Daniel
Unidade de Investigação Cardiovascular (UniC), Faculdade de Medicina da Universidade do Porto, Portugal.
Centro de Investigação em Atividade Física Saude e Lazer (CIAFEL), Faculdade de Desporto da Universidade do Porto, Portugal.
Porto Biomed J. 2020 Sep 16;5(5):e081. doi: 10.1097/j.pbj.0000000000000081. eCollection 2020 Sep-Oct.
Despite pulmonary arterial hypertension (PAH) directly affects the right ventricle (RV), important structural, functional, and molecular changes also occur in left ventricle (LV). The objective of our study was to analyze the hypothetical cardioprotective effects of exercise preconditioning on LV in rats with monocrotaline (MCT)-induced PAH.
Forty male Wistar rats were randomly separated in sedentary (SED) and trained group (EX; running sessions of 60 min/day, 5 days/wk, at 25 m/min, for 4 weeks). After 4 weeks, animals were injected with MCT (60 mg/kg; SED + MCT; EX + MCT) or vehicle (SED + V). Following an additional period of 4 weeks where all animals remained sedentary, we completed LV hemodynamic evaluation in baseline and isovolumic conditions and collected LV samples for histological and molecular analysis.
Preconditioning with exercise was capable to restore LV systolic and diastolic dysfunction in both baseline and isovolumic conditions ( < .05). This improved was paralleled with prevention of LV cardiomyocytes atrophy, fibrosis, and endothelin 1 mRNA levels ( < .05).
Our findings suggest that exercise preconditioning can prevent LV dysfunction secondary to MCT-induced PAH, which is of particular interest for the familial form of the disease that is manifested by greater severity or earlier onset.
尽管肺动脉高压（PAH）直接影响右心室（RV），但左心室（LV）也会发生重要的结构、功能和分子变化。我们研究的目的是分析运动预处理对用野百合碱（MCT）诱导的PAH大鼠左心室的假设性心脏保护作用。
40只雄性Wistar大鼠随机分为久坐组（SED）和训练组（EX；每天跑步60分钟，每周5天，速度为25米/分钟，共4周）。4周后，给动物注射MCT（60毫克/千克；SED + MCT；EX + MCT）或赋形剂（SED + V）。在所有动物继续保持久坐的另外4周后，我们在基线和等容条件下完成了左心室血流动力学评估，并收集左心室样本进行组织学和分子分析。
运动预处理能够在基线和等容条件下恢复左心室收缩和舒张功能障碍（P <.05）。这种改善与预防左心室心肌细胞萎缩、纤维化和内皮素1 mRNA水平平行（P <.05）。
我们的研究结果表明，运动预处理可以预防MCT诱导的PAH继发的左心室功能障碍，这对于以更严重或更早发作为特征的家族性疾病形式尤为重要。
