Nestmann E R, Brillinger R L, McPherson M F, Maus K L
Mutagenesis Section, Department of National Health and Welfare, Ottawa, Ontario, Canada.
Environ Mol Mutagen. 1987;10(2):169-81. doi: 10.1002/em.2850100207.
A new Salmonella mutagenicity test method is under development to test a chemical with more than one strain simultaneously (the "SIMULTEST"), that is, different Salmonella typhimurium tester strains are used in combination on the same plate. Strains are combined in two sets: strains with plasmid pKM101 (TA97, TA98, TA100, and TA102) and strains without the plasmid (TA1535, TA1537, and TA1538). The SIMULTEST combinations successfully detect the mutagenic activity of five mutagens in different chemical classes. This approach may be useful in reducing the workload associated with mutagenicity testing with Salmonella.
一种新的沙门氏菌致突变性测试方法正在研发中，用于同时对一种化学品进行多个菌株的测试（“同步测试”），即不同的鼠伤寒沙门氏菌测试菌株在同一平板上组合使用。菌株分为两组组合：携带质粒pKM101的菌株（TA97、TA98、TA100和TA102）和不携带质粒的菌株（TA1535、TA1537和TA1538）。同步测试组合成功检测了不同化学类别中五种诱变剂的诱变活性。这种方法可能有助于减少沙门氏菌致突变性测试的工作量。
