McPherson M F, Nestmann E R
Environmental Health Directorate, Department of National Health and Welfare, Ottawa, Ontario, Canada.
Environ Mol Mutagen. 1990;16(1):21-5. doi: 10.1002/em.2850160104.
The concept of combining several histidine-dependent Salmonella strains in a single test, the SIMULTEST, has been applied to the microtitre fluctuation test. The activity of five mutagens was determined in strains TA97, TA98, TA100, and TA102 individually as well as in a SIMULTEST mixture. All five compounds were mutagenic in the SIMULTEST, demonstrating the utility of this time and labour-saving approach of combining strains for testing with this method. The microtitre fluctuation SIMULTEST results were quantitatively comparable to those of the SIMULTEST Salmonella/microsome plate test. The microtitre fluctuation test compared with the plate incorporation assay generally showed more favourable "sensitivity" and "quantity" indices in that four of the five chemicals tested in the fluctuation test were mutagenic at lower doses than in the plate test.
将几种组氨酸依赖型沙门氏菌菌株组合在一次试验（即同步试验）中的概念已应用于微量滴定波动试验。分别在TA97、TA98、TA100和TA102菌株中以及在同步试验混合物中测定了五种诱变剂的活性。所有五种化合物在同步试验中均具有致突变性，证明了这种将菌株组合用于该方法测试的省时省力方法的实用性。微量滴定波动同步试验结果与同步试验沙门氏菌/微粒体平板试验结果在数量上具有可比性。与平板掺入试验相比，微量滴定波动试验通常显示出更有利的“敏感性”和“数量”指标，因为波动试验中测试的五种化学物质中有四种在比平板试验更低的剂量下具有致突变性。
