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静电擦拭布作为一种简单可靠的流感病毒空气传播检测方法。

Electrostatic wipes as simple and reliable methods for influenza virus airborne detection.

机构信息

Laboratoire de Virologie, CHU, Besançon, France.

EA3181, Université Bourgogne Franche-Comté, France.

出版信息

J Hosp Infect. 2021 Feb;108:15-18. doi: 10.1016/j.jhin.2020.10.030. Epub 2020 Nov 14.

Abstract

The performance of an in-house protocol for virus detection on commercialized electrostatic wipes (EWs) was assessed experimentally by impregnating them with suspensions of cytomegalovirus, adenovirus, and influenza virus, and by determining the recovery efficiency, repeatability, and detection limit of the protocol. The protocol was sensitive enough to detect 4 log gene copies of virus. At room temperature, influenza RNA was stable on EWs for at least four days. When EWs were placed high in 32 influenza-infected patients' rooms, influenza RNA was detectable in 75% (N = 24) of EWs, suggesting that EWs are simple and reliable methods for influenza virus airborne detection.

摘要

通过用巨细胞病毒、腺病毒和流感病毒的悬浮液浸渍商品化静电擦拭布(EWs),实验评估了一种内部病毒检测方案在该商用 EWs 上的性能,评估内容包括该方案的回收效率、重复性和检测限。该方案的灵敏度足以检测到 4 个对数级的病毒基因拷贝。在室温下,流感病毒 RNA 在 EWs 上至少稳定四天。当 EWs 被放置在 32 名流感感染患者的房间高处时,在 75%(N=24)的 EWs 中可检测到流感 RNA,这表明 EWs 是用于检测流感病毒空气传播的简单可靠的方法。

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