酵母中乙醇脱氢酶基因座的条件超显性
Conditional overdominance at an alcohol dehydrogenase locus in yeast.
作者信息
Hall J G, Wills C
机构信息
Department of Biology C-016, University of California at San Diego, La Jolla 92093.
出版信息
Genetics. 1987 Nov;117(3):421-7. doi: 10.1093/genetics/117.3.421.
Abstract
Documented examples of heterosis attributable to overdominance at specific protein-encoding gene loci have rarely been reported, the association of sickle cell hemoglobin with malarial resistance being the best documented example of this phenomenon. Here we report an example of overdominance that is temperature- and allyl alcohol-dependent and due to heterozygosity at the ADH1 locus, involving two ADHI functional mutants. Overdominance appears to be due in part to an intermediate level of ADHI activity in the heterozygote. Unlike previous work with this this system using haploid strains, the NAD+/NADH ratios show no negative correlation with allyl alcohol resistance. This system is formally equivalent to that of sickle cell hemoglobin and shows promise as a tool for investigating the physiological basis for overdominance.
摘要
归因于特定蛋白质编码基因座超显性的杂种优势的实例鲜有文献记载，镰状细胞血红蛋白与疟疾抗性的关联是该现象记载最为详尽的例子。在此，我们报告一个超显性的例子，它依赖于温度和烯丙醇，并且由于ADH1基因座的杂合性，涉及两个ADHI功能突变体。超显性似乎部分归因于杂合子中ADHI活性的中间水平。与之前使用单倍体菌株对该系统的研究不同，NAD+/NADH比率与烯丙醇抗性没有负相关。这个系统在形式上等同于镰状细胞血红蛋白系统，有望成为研究超显性生理基础的工具。
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本文引用的文献
1
Hybrid protein formation of E. coli alkaline phosphatase leading to in vitro complementation.导致体外互补的大肠杆菌碱性磷酸酶的杂合蛋白形成。
J Mol Biol. 1963 Jul;7:1-12. doi: 10.1016/s0022-2836(63)80014-5.
2
The molecular basis of dominance.显性的分子基础。
Genetics. 1981 Mar-Apr;97(3-4):639-66. doi: 10.1093/genetics/97.3-4.639.
3
Mutants of Aerobacter aerogenes capable of utilizing xylitol as a novel carbon.产气气杆菌中能够利用木糖醇作为新型碳源的突变体。
J Bacteriol. 1968 Aug;96(2):447-56. doi: 10.1128/jb.96.2.447-456.1968.
4
Gene duplication in experimental enzyme evolution.实验性酶进化中的基因复制
Nature. 1974 Sep 20;251(5472):200-4. doi: 10.1038/251200a0.
5
Allyl alcohol-induced irreversible inhibition of yeast alcohol dehydrogenase.烯丙醇对酵母乙醇脱氢酶的不可逆抑制作用。
Biochem Pharmacol. 1974 Aug 15;23(16):2328-31. doi: 10.1016/0006-2952(74)90563-2.
6
The control of flux.通量的控制
Symp Soc Exp Biol. 1973;27:65-104.
7
Partial correction of structural defects in alcohol dehydrogenase through interallelic complementation in Drosophila melanogaster.通过黑腹果蝇中的等位基因间互补对乙醇脱氢酶结构缺陷进行部分校正。
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8
Amino acid substitutions in two functional mutants of yeast alcohol dehydrogenase.
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9
Protein complementation.蛋白质互补作用
Annu Rev Biochem. 1975;44:295-313. doi: 10.1146/annurev.bi.44.070175.001455.
10
Quantitative extraction and estimation of intracellular nicotinamide nucleotides of Escherichia coli.
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