Cellular and Molecular Biotechnology Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), 1-1-1 Higashi, Tsukuba, Ibaraki, 305-8565, Japan.
Department of Mechanism of Aging, Research Institute, National Center for Geriatrics and Gerontology, Obu, Aichi 474-8511, Japan.
Arch Oral Biol. 2021 Jan;121:104967. doi: 10.1016/j.archoralbio.2020.104967. Epub 2020 Nov 5.
Mucins are large glycosylated glycoproteins that are produced in the salivary glands, and their changes may contribute to the development of xerostomia due to aging and the accompanying deterioration of oral hygiene. This study aimed to characterize the changes in the mucins produced in submandibular gland (SMG) during the aging process.
SMG mucins derived from mice of each age were separated using supported molecular matrix electrophoresis (SMME). Subsequently, the membranes were stained with Alcian blue (AB) or blotted with MAL-II lectin. The SMME membranes stained with AB were subjected to densitometric analysis and glycan analysis. The detailed structures of O-glycan were investigated by tandem mass spectrometry (MS/MS).
The SMG of mice secreted three mucins with different glycan profiles: age-specific mucin, youth-specific mucin, and a mucin expressed throughout life, and the expression patterns of these mucins change during aging. Additionally, age-specific mucin began to be detected at about 12 months of age. A mucin expressed throughout life and age-specific mucin had the same mass of major glycans but different structures. Furthermore, the proportion of mucin glycan species expressed throughout life changed during the aging process, and aging tended to decrease the proportion of fucosylated glycans and increase the proportion of sialoglycans.
There are three secretory mucins with different glycan profiles in the SMG of mice, and their expression patterns change according to the period of the aging process. The proportion of glycan species of mucin expressed throughout life also changes during the aging process.
黏蛋白是一种在唾液腺中产生的大型糖基化糖蛋白，其变化可能导致衰老和随之而来的口腔卫生恶化导致的口干。本研究旨在描述下颌下腺 (SMG) 中黏蛋白在衰老过程中的变化。
使用支持分子基质电泳 (SMME) 分离来自每个年龄组小鼠的 SMG 黏蛋白。随后，用 Alcian blue (AB) 染色或 MAL-II 凝集素印迹膜。用 AB 染色的 SMME 膜进行密度分析和聚糖分析。通过串联质谱 (MS/MS) 研究 O-聚糖的详细结构。
小鼠的 SMG 分泌了三种具有不同聚糖谱的黏蛋白：年龄特异性黏蛋白、青年特异性黏蛋白和终生表达的黏蛋白，这些黏蛋白的表达模式在衰老过程中发生变化。此外，年龄特异性黏蛋白大约在 12 个月大时开始被检测到。终生表达的黏蛋白和年龄特异性黏蛋白具有相同的主要糖质量，但结构不同。此外，终生表达的黏蛋白聚糖种类的比例在衰老过程中发生变化，衰老倾向于降低岩藻糖基化聚糖的比例并增加唾液酸化聚糖的比例。
在小鼠的 SMG 中有三种具有不同聚糖谱的分泌性黏蛋白，它们的表达模式根据衰老过程的时期而变化。终生表达的黏蛋白聚糖种类的比例也在衰老过程中发生变化。
