Division of Oral and Maxillofacial Surgery, Ushiku Aiwa General Hospital, Ushiku, Japan.
Department of Oral Oncology, Oral and Maxillofacial Surgery, Ichikawa General Hospital, Tokyo Dental College, Ichikawa, Japan.
Methods Mol Biol. 2024;2763:45-50. doi: 10.1007/978-1-0716-3670-1_3.
Studying salivary gland mucins is important for elucidating the pathogenesis of salivary gland diseases, including tumors and xerostomia, and developing diagnostic methods for them. Classic methods for isolating mucins from salivary glands require sacrificing several animals to obtain sufficient quantities of mucin and are time-consuming. Supported molecular matrix electrophoresis (SMME) was used to characterize mucins and their glycans. With this method, mucins can be analyzed within 2 days using less than 100 mg of tissue and without using expensive equipment, such as an ultracentrifuge. This chapter describes a method for preparing mucin solutions for SMME analysis of salivary gland mucins.
研究唾液腺黏蛋白对于阐明唾液腺疾病（包括肿瘤和口干症）的发病机制以及开发这些疾病的诊断方法非常重要。从唾液腺中分离黏蛋白的经典方法需要牺牲几只动物以获得足够数量的黏蛋白，而且耗时较长。支持分子基质电泳（SMME）被用于分析黏蛋白及其聚糖。使用这种方法，只需要不到 100 毫克的组织和不需要使用超速离心机等昂贵设备，就可以在 2 天内分析黏蛋白。本章描述了一种用于制备 SMME 分析唾液腺黏蛋白的黏蛋白溶液的方法。
