Serag Ahmed, Salem Mohamed A, Gong Shilin, Wu Jian-Lin, Farag Mohamed A
Pharmaceutical Analytical Chemistry Department, Faculty of Pharmacy, Al-Azhar University, Cairo 11751, Egypt.
Department of Pharmacognosy and Natural Products, Faculty of Pharmacy, Menoufia University, Gamal Abd El Nasr st., Shibin Elkom 32511, Menoufia, Egypt.
Metabolites. 2023 Mar 13;13(3):424. doi: 10.3390/metabo13030424.
In their environment, plants interact with a multitude of living organisms and have to cope with a large variety of aggressions of biotic or abiotic origin. What has been known for several decades is that the extraordinary variety of chemical compounds the plants are capable of synthesizing may be estimated in the range of hundreds of thousands, but only a fraction has been fully characterized to be implicated in defense responses. Despite the vast importance of these metabolites for plants and also for human health, our knowledge about their biosynthetic pathways and functions is still fragmentary. Recent progress has been made particularly for the phenylpropanoids and oxylipids metabolism, which is more emphasized in this review. With an increasing interest in monitoring plant metabolic reprogramming, the development of advanced analysis methods should now follow. This review capitalizes on the advanced technologies used in metabolome mapping in planta, including different metabolomics approaches, imaging, flux analysis, and interpretation using bioinformatics tools. Advantages and limitations with regards to the application of each technique towards monitoring which metabolite class or type are highlighted, with special emphasis on the necessary future developments to better mirror such intricate metabolic interactions in .
在其环境中，植物与众多生物相互作用，必须应对各种生物或非生物来源的侵害。几十年来人们已经知道，植物能够合成的化合物种类异常繁多，估计可达数十万种，但只有一小部分已被充分表征与防御反应有关。尽管这些代谢物对植物和人类健康都极为重要，但我们对其生物合成途径和功能的了解仍然支离破碎。最近在苯丙烷类和氧脂类代谢方面取得了进展，本综述将更加强调这一点。随着对监测植物代谢重编程的兴趣日益增加，现在应该跟进先进分析方法的发展。本综述利用了用于植物代谢组图谱绘制的先进技术，包括不同的代谢组学方法、成像、通量分析以及使用生物信息学工具进行的解释。文中强调了每种技术在监测哪些代谢物类别或类型方面应用的优缺点，并特别强调了未来为更好地反映植物中如此复杂的代谢相互作用而必须进行的发展。
